biostudies-literatureQi GNFundamental Research Funds for the Central Universities of ChinaNatural Science Foundation of ShanghaiNational Natural Science Foundation of China1192-1208https://www.ebi.ac.uk/biostudies/studies/S-EPMC11821748biostudies-literatureUnknown197(2)A new lipopeptide-producing strain Cytobacillus sp. R3-1 was isolated from the production water of the Daqing oilfield in China and identified based on 16S rRNA gene sequence analyses. The strain R3-1 is capable of simultaneously producing both of the surfactin and fengycin, the two major families of the lipopeptide biosurfactant. The chemical structures of the surfactin and fengycin were confirmed by a combination of the ESI-MS, FT-IR, and amino acid analyses, and the impact of various temperatures, pH, and NaCl concentrations on the emulsifying activity (E₂₄) was investigated. The lipopeptide biosurfactant produced by the strain R3-1 exhibited strong emulsifying activity with E₂₄ value over 60% on crude oil and different hydrocarbons, including the cyclohexane, hexaApplied biochemistry and biotechnologyA New Bacterial Strain Producing Both of the Surfactin and Fengycin Lipopeptide Biosurfactant with Strong Emulsifications on Crude Oil.PMC11821748JKJ0124171421ZR141740042061134011 and 42173076Zhou LGang HZMu BZMa TTQin WQLiu YFYang SZLi GJLiu JFQi GNfalseA New Bacterial Strain Producing Both of the Surfactin and Fengycin Lipopeptide Biosurfactant with Strong Emulsifications on Crude Oil.A new lipopeptide-producing strain Cytobacillus sp. R3-1 was isolated from the production water of the Daqing oilfield in China and identified based on 16S rRNA gene sequence analyses. The strain R3-1 is capable of simultaneously producing both of the surfactin and fengycin, the two major families of the lipopeptide biosurfactant. The chemical structures of the surfactin and fengycin were confirmed by a combination of the ESI-MS, FT-IR, and amino acid analyses, and the impact of various temperatures, pH, and NaCl concentrations on the emulsifying activity (E₂₄) was investigated. The lipopeptide biosurfactant produced by the strain R3-1 exhibited strong emulsifying activity with E₂₄ value over 60% on crude oil and different hydrocarbons, including the cyclohexane, hexa2025-01-01T00:00:00Z2025 Feb2025-04-26T16:36:58.162Z2025-04-06T15:13:33.598ZS-EPMC118217483940499810.1007/s12010-024-05076-1