biostudies-literatureUnknown25(1)Liao F<h4>Background</h4>Pulsed electromagnetic fields (PEMFs) therapy was extensively investigated to treat wound healing, which is a highly metabolically demanding process. However, the effect of PEMFs on energy metabolism in wound healing remains largely unexplored. Therefore, our study aims to demonstrate the role of PEMFs on energy metabolism in wound healing.<h4>Methods</h4>Scratch-wound healing assay and cell viability assay were performed for the in vitro study of the effect of PEMFs on cell migration and viability. Seahorse assay was conducted for energy metabolism analysis while holo-tomographic microscopy for fine changes of L929 cells. Mitochondrial membrane potential assay and intracellular reactive oxygen species (ROS) and pH assay were performed for analyzing the changes of mitochBMC complementary medicine and therapies50https://www.ebi.ac.uk/biostudies/studies/S-EPMC11823022biostudies-literaturePulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.PMC11823022Liu HLi YLiao FZhang ZYu QfalsePulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.<h4>Background</h4>Pulsed electromagnetic fields (PEMFs) therapy was extensively investigated to treat wound healing, which is a highly metabolically demanding process. However, the effect of PEMFs on energy metabolism in wound healing remains largely unexplored. Therefore, our study aims to demonstrate the role of PEMFs on energy metabolism in wound healing.<h4>Methods</h4>Scratch-wound healing assay and cell viability assay were performed for the in vitro study of the effect of PEMFs on cell migration and viability. Seahorse assay was conducted for energy metabolism analysis while holo-tomographic microscopy for fine changes of L929 cells. Mitochondrial membrane potential assay and intracellular reactive oxygen species (ROS) and pH assay were performed for analyzing the changes of mitoch2025-01-01T00:00:00Z2025 Feb2025-04-04T23:56:43.817Z2025-04-04T23:56:43.817ZS-EPMC118230223993986610.1186/s12906-025-04792-3