biostudies-literaturePetrozziello TNIA NIH HHSNINDS NIH HHS267-281https://www.ebi.ac.uk/biostudies/studies/S-EPMC11823699biostudies-literatureUnknown12(3)<h4>Background</h4>To date, it is still controversial whether tau phosphorylation plays a role in Huntington's disease (HD), as previous studies demonstrated either no alterations or increases in phosphorylated tau (pTau) in HD postmortem brain and mouse models.<h4>Objective</h4>The goal of this study was to determine whether total tau and pTau levels are altered in HD.<h4>Methods</h4>Immunohistochemistry, cellular fractionations, and western blots were used to measure total tau and pTau levels in a large cohort of HD and control postmortem prefrontal cortex (PFC). Furthermore, western blots were performed to assess tau, and pTau levels in HD and control isogenic embryonic stem cell (ESC)-derived cortical neurons and neuronal stem cells (NSCs). Similarly, western blots were used to assess Journal of Huntington's diseaseAge-Dependent Increase in Tau Phosphorylation at Serine 396 in Huntington's Disease Prefrontal Cortex.PMC11823699R01 NS126420P30 AG062421Connors TRWu MQuinn JPPouladi MAHuntress SSDiFiglia MSunderesh SRRosas HDBoudi ALiu SPetrozziello TMills ANMouro Pinto RMahmood FSadri-Vakili GCastillo-Torres ALAuger CAArnold SEKim SEHyman BTKivisakk PKegel-Gleason KSapp EfalseAge-Dependent Increase in Tau Phosphorylation at Serine 396 in Huntington's Disease Prefrontal Cortex.<h4>Background</h4>To date, it is still controversial whether tau phosphorylation plays a role in Huntington's disease (HD), as previous studies demonstrated either no alterations or increases in phosphorylated tau (pTau) in HD postmortem brain and mouse models.<h4>Objective</h4>The goal of this study was to determine whether total tau and pTau levels are altered in HD.<h4>Methods</h4>Immunohistochemistry, cellular fractionations, and western blots were used to measure total tau and pTau levels in a large cohort of HD and control postmortem prefrontal cortex (PFC). Furthermore, western blots were performed to assess tau, and pTau levels in HD and control isogenic embryonic stem cell (ESC)-derived cortical neurons and neuronal stem cells (NSCs). Similarly, western blots were used to assess 2023-01-01T00:00:00Z20232025-04-05T09:39:26.604Z2025-04-05T09:39:26.604ZS-EPMC118236993769437210.3233/JHD-23058810.3233/jhd-230588