biostudies-literatureMontico BAssociazione Italiana per la Ricerca sul CancroMinistero della Salute53https://www.ebi.ac.uk/biostudies/studies/S-EPMC11827140biostudies-literatureUnknown44(1)<h4>Background</h4>About 50% of cutaneous melanoma (CM) harbors the activating BRAF^V600 mutation which exerts most of the oncogenic effects through the MAPK signaling pathway. In the last years, a number of MAPK modulators have been identified, including Spry1. In this context, we have recently demonstrated that knockout of Spry1 (Spry1^KO) in BRAF^V600-mutant CM led to cell cycle arrest and apoptosis, repressed cell proliferation in vitro, and reduced tumor growth in vivo. Despite these findings, however, the precise molecular mechanism linking Spry1 to BRAF^V600-mutant CM remains to be elucidated.<h4>Materials and methods</h4>Immunoprecipitation coupled to mass spectrometry was employed to gain insight into Spry1 interactome. Spry1 gene was knockeJournal of experimental & clinical cancer research : CRSuppression of Spry1 reduces HIF1α-dependent glycolysis and impairs angiogenesis in BRAF-mutant cutaneous melanoma.PMC11827140IG-23068GR-2018-12366312RF-2018-12365425IG-23643Giurato GCovre ASigalotti LMontico BLamberti JComelli MNassa GMemoli DFratta EGuerrieri RNyman TAMaio MAndreuzzi EColizzi FFejza ABaboci LDal Bo MSalvati AMongiat MSabatelli PSteffan AWeisz ABellazzo ACamicia LfalseSuppression of Spry1 reduces HIF1α-dependent glycolysis and impairs angiogenesis in BRAF-mutant cutaneous melanoma.<h4>Background</h4>About 50% of cutaneous melanoma (CM) harbors the activating BRAF^V600 mutation which exerts most of the oncogenic effects through the MAPK signaling pathway. In the last years, a number of MAPK modulators have been identified, including Spry1. In this context, we have recently demonstrated that knockout of Spry1 (Spry1^KO) in BRAF^V600-mutant CM led to cell cycle arrest and apoptosis, repressed cell proliferation in vitro, and reduced tumor growth in vivo. Despite these findings, however, the precise molecular mechanism linking Spry1 to BRAF^V600-mutant CM remains to be elucidated.<h4>Materials and methods</h4>Immunoprecipitation coupled to mass spectrometry was employed to gain insight into Spry1 interactome. Spry1 gene was knocke2025-01-01T00:00:00Z2025 Feb2025-04-18T13:03:36.671Z2025-04-04T02:26:14.407ZS-EPMC118271403995361010.1186/s13046-025-03289-8