biostudies-literatureXiong Nthe project of "Dengfeng plan" from Foshan Hospital of Traditional Chinese MedicineBeijing Xisike Clinical Oncology Research FoundationNational Natural Science Foundation of ChinaBeijing Bethune Charitable FoundationNational Natural Science Foundation of ChongqingBeijing Natural Science FoundationNational Key Research and Development of Chinae2411740https://www.ebi.ac.uk/biostudies/studies/S-EPMC11831497biostudies-literatureUnknown12(7)Liver metastasis is the primary factor contributing to unfavorable prognosis in colorectal cancer (CRC). Although N-glycosylation is implicated in metastasis, there is a notable paucity of comprehensive studies addressing the N-glycosylation proteomics associated with liver metastasis in CRC. In this study, N-glycosylated proteins and N-glycosylation sites of differential expression between primary lesions and paired liver metastatic lesions are identified. Cathepsin D (CTSD) is further screened as a potentially pivotal N-glycosylated protein in CRC liver metastasis. Glycosyltransferases complex DDOST and STT3B can regulate N-glycosylation modification at residue 263 of CTSD (a protease), thereby affecting CTSD protease to lyse ACADM. ACADM can regulate ferroptosis-related proteins (ACSL4,Advanced science (Weinheim, Baden-Wurttemberg, Germany)N-glycosylation Modification of CTSD Affects Liver Metastases in Colorectal Cancer.PMC11831497Y-bayer202001-01222022YFC34008012159000087Z220014718217182272841620724352020002059247811782170801CSTB2023NSCQ-MSX0131Huang CYan QXiong NWang CZhang HDu YYe YYang CLi YZhao LShen ZSun QWang SGao ZZhan JfalseN-glycosylation Modification of CTSD Affects Liver Metastases in Colorectal Cancer.Liver metastasis is the primary factor contributing to unfavorable prognosis in colorectal cancer (CRC). Although N-glycosylation is implicated in metastasis, there is a notable paucity of comprehensive studies addressing the N-glycosylation proteomics associated with liver metastasis in CRC. In this study, N-glycosylated proteins and N-glycosylation sites of differential expression between primary lesions and paired liver metastatic lesions are identified. Cathepsin D (CTSD) is further screened as a potentially pivotal N-glycosylated protein in CRC liver metastasis. Glycosyltransferases complex DDOST and STT3B can regulate N-glycosylation modification at residue 263 of CTSD (a protease), thereby affecting CTSD protease to lyse ACADM. ACADM can regulate ferroptosis-related proteins (ACSL4,2025-01-01T00:00:00Z2025 Feb2025-04-04T01:24:17.26Z2025-04-04T01:24:17.26ZS-EPMC118314973971692710.1002/advs.202411740