biostudies-literatureUnknown15(7)Kornsakulkarn JEntomopathogenic fungi of the genus Hypocrella are known to produce bioactive compounds, in particular polyketides. Here, a chemical investigation of the entomopathogenic fungus <i>Hypocrella luteola</i> strain TBRC-BCC 76666 was performed to identify possible novel compounds indicated from HPLC and ¹H NMR spectra profiles of culture extracts. Eight novel compounds were isolated, including six new polyketides (1-6) and two other naturally occurring compounds (7-8), along with seven known compounds. The compound structures were established by spectroscopic analysis, the application of modified Mosher's method, and electronic circular dichroism. Compounds 1 and 5 were cytotoxic against both NCI-H187 and Vero cells (IC₅₀ = 9.9 and 58.9 μM, respectively), whereas compound 4 was active only against MCF-7 cells (IC₅₀ = 42.5 μM). Ascherxanthone B (9) exhibited antiproliferative activity against all cell lines tested (<i>Plasmodium falciparum</i>, mammalian, and fungal), with IC₅₀ and MIC values in the range 0.9-30.5 μM.RSC advances5234-5241https://www.ebi.ac.uk/biostudies/studies/S-EPMC11831733biostudies-literatureBioactive polyketides from cultures of the entomopathogenic fungus <i>Hypocrella luteola</i> TBRC-BCC 76666.PMC11831733Thongpanchang CMongkolsamrit SKornsakulkarn JAuncharoen PNoisripoom WfalseBioactive polyketides from cultures of the entomopathogenic fungus <i>Hypocrella luteola</i> TBRC-BCC 76666.Entomopathogenic fungi of the genus Hypocrella are known to produce bioactive compounds, in particular polyketides. Here, a chemical investigation of the entomopathogenic fungus <i>Hypocrella luteola</i> strain TBRC-BCC 76666 was performed to identify possible novel compounds indicated from HPLC and ¹H NMR spectra profiles of culture extracts. Eight novel compounds were isolated, including six new polyketides (1-6) and two other naturally occurring compounds (7-8), along with seven known compounds. The compound structures were established by spectroscopic analysis, the application of modified Mosher's method, and electronic circular dichroism. Compounds 1 and 5 were cytotoxic against both NCI-H187 and Vero cells (IC₅₀ = 9.9 and 58.9 μM, respectively), whereas compound 4 was active only against MCF-7 cells (IC₅₀ = 42.5 μM). Ascherxanthone B (9) exhibited antiproliferative activity against all cell lines tested (<i>Plasmodium falciparum</i>, mammalian, and fungal), with IC₅₀ and MIC values in the range 0.9-30.5 μM.2025-01-01T00:00:00Z2025 Feb2026-06-01T10:30:13.99Z2025-04-04T02:01:31.649ZS-EPMC118317333996345910.1039/d4ra08431d