{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["71(8)"],"submitter":["Mills S"],"pubmed_abstract":["Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones."],"journal":["Applied and environmental microbiology"],"pagination":["4897-901"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1183277"],"repository":["biostudies-literature"],"pubmed_title":["Insertional inactivation of determinants for Mg2+ and Co2+ transport as a tool for screening recombinant Lactococcus species clones."],"pmcid":["PMC1183277"],"pubmed_authors":["Hill C","Ross RP","McAuliffe O","Mills S","Coffey A","Fitzgerald GF"],"additional_accession":[]},"is_claimable":false,"name":"Insertional inactivation of determinants for Mg2+ and Co2+ transport as a tool for screening recombinant Lactococcus species clones.","description":"Insertional inactivation of the plasmid-encoded determinants for Mg(2+) and Co(2+) transport, orf18/corA, provides a tool for screening recombinant clones in Lactococcus, based on the observation that overexpression of orf18/corA results in cell growth inhibition on certain concentrations of CoCl(2). The lacticin 3147 immunity gene, ltnI, was used to insertionally inactivate orf18/corA. The resulting clones were capable of growth on concentrations of CoCl(2) that were inhibitory to the parent strain. Since only 3 of 17 lactococcal starters naturally harbor corA, the system has potential as a screen for selecting recombinant lactococcal clones.","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Aug","modification":"2025-04-04T21:07:09.361Z","creation":"2019-03-27T01:09:13Z"},"accession":"S-EPMC1183277","cross_references":{"pubmed":["16085892"],"doi":["10.1128/AEM.71.8.4897-4901.2005","10.1128/aem.71.8.4897-4901.2005"]}}