biostudies-literatureUnknown9Cadenas-Jimenez I<h4>Background</h4><i>Pseudomonas aeruginosa</i> chronic lung infection is the leading cause of death in the cystic fibrosis (CF) population. The high genome versatility of this microorganism allows it to adapt to the hostile CF lung where the same clone can persist for decades. Paranasal sinuses serve as a reservoir for bacterial adaptation before lung infection. Our study investigates biofilm compatibility among identical and different <i>P. aeruginosa</i> genotypes from sinus and lungs of CF patients. Strains were further characterized by whole genome sequencing and motility assays were performed.<h4>Methodology</h4>Motility, gentamicin susceptibility and growth rates were assessed in four strains coming from three CF patients. The strains were subjected to whole genome sequencing with Biofilm100257https://www.ebi.ac.uk/biostudies/studies/S-EPMC11834076biostudies-literatureCo-culture biofilm patterns among different Pseudomonas aeruginosa clones from cystic fibrosis patients.PMC11834076Hoiby NHigazy DCadenas-Jimenez IRybtke MLMarti-Marti STolker-Nielsen TCiofu OfalseCo-culture biofilm patterns among different Pseudomonas aeruginosa clones from cystic fibrosis patients.<h4>Background</h4><i>Pseudomonas aeruginosa</i> chronic lung infection is the leading cause of death in the cystic fibrosis (CF) population. The high genome versatility of this microorganism allows it to adapt to the hostile CF lung where the same clone can persist for decades. Paranasal sinuses serve as a reservoir for bacterial adaptation before lung infection. Our study investigates biofilm compatibility among identical and different <i>P. aeruginosa</i> genotypes from sinus and lungs of CF patients. Strains were further characterized by whole genome sequencing and motility assays were performed.<h4>Methodology</h4>Motility, gentamicin susceptibility and growth rates were assessed in four strains coming from three CF patients. The strains were subjected to whole genome sequencing with 2025-01-01T00:00:00Z2025 Jun2026-07-15T08:46:11.033Z2025-04-04T02:12:41.894ZS-EPMC118340763996837510.1016/j.bioflm.2025.100257