{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Zhang Z"],"funding":["NIA NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 are common in multiple types of human cancer, leading to the accumulation of D-2-hydroxyglutarate (D2HG) and the promotion of tumorigenesis<sup>1</sup>. Here we discovered a novel <i>O</i>-2-hydroxyglutarylation by D2HG using chemical proteomics and further revealed distinct chiral preferences for D/L2HG modifications. Notably, we identified two kinases, MRCKA and SLK, modified by D2HG and L2HG respectively, and detected reduced phosphorylation of their substrates, suggesting an inhibitory effect of D/L 2HG modifications on the kinases' activity."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.01.24.634716"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11838245"],"repository":["biostudies-literature"],"pubmed_title":["Discovery of Chirally-dependent Protein <i>O</i>-2-Hydroxyglutarylation by D2HG and L2HG."],"pmcid":["PMC11838245"],"funding_grant_id":["R01 AG064250","P30 CA023168","R01 CA069202","R35 GM138002"],"pubmed_authors":["Wu MJ","Xue F","Bardeesy N","Zhang Z","Luo Z","Zhang ZY","Liu YK","Qu Z","Tao WA","Parkinson EI","Evans CN"],"additional_accession":[]},"is_claimable":false,"name":"Discovery of Chirally-dependent Protein <i>O</i>-2-Hydroxyglutarylation by D2HG and L2HG.","description":"Mutations in isocitrate dehydrogenase 1 (IDH1) and IDH2 are common in multiple types of human cancer, leading to the accumulation of D-2-hydroxyglutarate (D2HG) and the promotion of tumorigenesis<sup>1</sup>. Here we discovered a novel <i>O</i>-2-hydroxyglutarylation by D2HG using chemical proteomics and further revealed distinct chiral preferences for D/L2HG modifications. Notably, we identified two kinases, MRCKA and SLK, modified by D2HG and L2HG respectively, and detected reduced phosphorylation of their substrates, suggesting an inhibitory effect of D/L 2HG modifications on the kinases' activity.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2025-04-18T13:52:53.446Z","creation":"2025-04-06T23:41:31.536Z"},"accession":"S-EPMC11838245","cross_references":{"pubmed":["39975355"],"doi":["10.1101/2025.01.24.634716"]}}