{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Julian J"],"funding":["Austrian Science Fund FWF","European Research Council","Vienna Science and Technology Fund (Wiener Wissenschafts-, Forschungs- und Technologiefonds)","EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)"],"pagination":["321-339"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11842276"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(2)"],"pubmed_abstract":["Vacuoles are essential for cellular metabolism and growth and the maintenance of internal turgor pressure. They sequester lytic enzymes, ions and secondary metabolites that, if leaked into the cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved vacuolar quality control pathway that is activated upon cell wall damage in a turgor-pressure-dependent manner. Cell wall perturbations induce a distinct modification-ATG8ylation-on the vacuolar membrane (tonoplast) that is regulated by the V-ATPase and ATG8 conjugation machinery. Genetic disruption of tonoplast ATG8ylation impairs vacuolar integrity, leading to cell death. Together, our findings reveal a homeostatic p"],"journal":["Nature plants"],"pubmed_title":["ATG8ylation of vacuolar membrane protects plants against cell wall damage."],"pmcid":["PMC11842276"],"funding_grant_id":["101000981","P34944","Marie Curie Fellowship Project number: 101107472","Project number: 101043370","ESP 580","Marie Curie VIP2 Program","LS21-009","P32355","project number: 101000981","101043370"],"pubmed_authors":["Bachmair A","Sprakel J","Besten M","Dank C","Andersen TG","Carlos De La Concepcion J","Julian J","Grujic N","Korbei B","Gao P","Del Chiaro A","Somssich M","Clavel M","Kobylinska R","Coll NS","Minina EA","Armengot L","Polivka I","Dagdas Y","Duverge H"],"additional_accession":[]},"is_claimable":false,"name":"ATG8ylation of vacuolar membrane protects plants against cell wall damage.","description":"Vacuoles are essential for cellular metabolism and growth and the maintenance of internal turgor pressure. They sequester lytic enzymes, ions and secondary metabolites that, if leaked into the cytosol, could lead to cell death. Despite their pivotal roles, quality control pathways that safeguard vacuolar integrity have remained elusive in plants. Here we describe a conserved vacuolar quality control pathway that is activated upon cell wall damage in a turgor-pressure-dependent manner. Cell wall perturbations induce a distinct modification-ATG8ylation-on the vacuolar membrane (tonoplast) that is regulated by the V-ATPase and ATG8 conjugation machinery. Genetic disruption of tonoplast ATG8ylation impairs vacuolar integrity, leading to cell death. Together, our findings reveal a homeostatic p","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2025-04-18T12:53:44.36Z","creation":"2025-04-04T00:51:49.057Z"},"accession":"S-EPMC11842276","cross_references":{"pubmed":["39920307"],"doi":["10.1038/s41477-025-01907-z"]}}