biostudies-literatureUnknownVallmitjana ANIBIB NIH HHSNCI NIH HHSNIAMS NIH HHSGSLab addresses the need for effective image analysis tools in fluorescence microscopy by providing an open-source platform that enhances traditional phasor analysis with advanced features. Key capabilities include machine learning-based clustering, real-time monitoring, and quantitative unmixing of fluorescent species. Designed for both commercial and custom systems, GSLab provides researchers with comprehensive lifetime and spectral phasor image analysis tools to tackle complex biological problems.bioRxiv : the preprint server for biology2025.02.10.637545https://www.ebi.ac.uk/biostudies/studies/S-EPMC11844366biostudies-literatureGSLab: Open-Source Platform for Advanced Phasor Analysis in Fluorescence Microscopy.PMC11844366R01 EB026705P30 AR075047R01 CA259019R21 AR082648Vallmitjana ARajil NRanjit STorrado BDurkin AFBalu MDvornikov AfalseGSLab: Open-Source Platform for Advanced Phasor Analysis in Fluorescence Microscopy.GSLab addresses the need for effective image analysis tools in fluorescence microscopy by providing an open-source platform that enhances traditional phasor analysis with advanced features. Key capabilities include machine learning-based clustering, real-time monitoring, and quantitative unmixing of fluorescent species. Designed for both commercial and custom systems, GSLab provides researchers with comprehensive lifetime and spectral phasor image analysis tools to tackle complex biological problems.2025-01-01T00:00:00Z2025 Feb2025-04-04T00:26:29.885Z2025-04-04T00:26:29.885ZS-EPMC118443663999034410.1101/2025.02.10.637545