{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Tang Q"],"funding":["National Center for Advancing Translational Sciences","NCATS NIH HHS","NIDCR NIH HHS","National Institute of Dental and Craniofacial Research"],"pubmed_abstract":["Tooth enamel, the hardest vertebrate tissue, is crucial for mastication and dental protection. Its formation depends on the enamel organ (EO), a specialized epithelial structure derived from oral epithelium. How uniform oral epithelium differentiates into diverse EO cell types remains unclear. While <i>p63</i>, an ectodermal development master regulator, is essential for dental placode formation, its specific roles in EO development have been obscured by early arrest in p63 knockout mice. Using single-cell RNA sequencing from mouse incisors, we show <i>p63</i> expression across all EO cell types with both shared and distinct functions. Through trajectory reconstruction, we identify <i>p63</i>'s role in regulating both amelogenic (AmG) and non-AmG lineage commitment during EO development. C"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.02.11.637463"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11844444"],"repository":["biostudies-literature"],"pubmed_title":["p63 co-opts the skin Krt8-to-Krt5 transition for enamel organ development."],"pmcid":["PMC11844444"],"funding_grant_id":["T32DE023526","KL2TR001413","R03 DE030985","UL1TR001412","T32 DE023526","R01 DE033085","UL1 TR001412","R03DE030985","R01DE033085","KL2 TR001413"],"pubmed_authors":["Kwon HE","Cai C","Tang Q","Jung H","Lee JM","Li L"],"additional_accession":[]},"is_claimable":false,"name":"p63 co-opts the skin Krt8-to-Krt5 transition for enamel organ development.","description":"Tooth enamel, the hardest vertebrate tissue, is crucial for mastication and dental protection. Its formation depends on the enamel organ (EO), a specialized epithelial structure derived from oral epithelium. How uniform oral epithelium differentiates into diverse EO cell types remains unclear. While <i>p63</i>, an ectodermal development master regulator, is essential for dental placode formation, its specific roles in EO development have been obscured by early arrest in p63 knockout mice. Using single-cell RNA sequencing from mouse incisors, we show <i>p63</i> expression across all EO cell types with both shared and distinct functions. Through trajectory reconstruction, we identify <i>p63</i>'s role in regulating both amelogenic (AmG) and non-AmG lineage commitment during EO development. C","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Apr","modification":"2026-07-03T03:23:03.69Z","creation":"2025-04-04T01:12:42.703Z"},"accession":"S-EPMC11844444","cross_references":{"pubmed":["39990386"],"doi":["10.1101/2025.02.11.637463"]}}