{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Sanguankiattichai N"],"funding":["European Research Council","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pubmed_abstract":["The extracellular space (apoplast) of plants is an important molecular battleground during infection by many pathogens. We previously found that a plant-secreted β-galactosidase BGAL1 acts in immunity by facilitating the release of immunogenic peptides from bacterial flagellin and that <i>Pseudomonas syringae</i> suppresses this enzyme by producing a small molecule inhibitor called galactosyrin. Here, we elucidated the structure and biosynthesis of galactosyrin and uncovered its multifunctional roles during infection. Structural elucidation by cryo-EM and chemical synthesis revealed that galactosyrin is an iminosugar featuring a unique geminal diol attached to the pyrrolidine moiety that mimics galactose binding to the β-galactosidase active site. Galactosyrin biosynthesis branches off fro"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.02.13.638044"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11844564"],"repository":["biostudies-literature"],"pubmed_title":["Bacterial pathogen deploys iminosugar galactosyrin to manipulate plant glycobiology."],"pmcid":["PMC11844564"],"funding_grant_id":["203141/Z/16/Z","BB/R017913/1","206422/Z/17/Z","101019324","BB/T015128/1"],"pubmed_authors":["Drapal M","Sheng Y","Tabak WWA","Chandrasekar B","Krahn D","Yamamoto S","Fleet G","van der Hoorn RAL","Zhang P","Preston GM","Hardenbrook N","Fraser P","Kato A","Sanguankiattichai N","Nash R","Buscaill P","Kaiser M"],"additional_accession":[]},"is_claimable":false,"name":"Bacterial pathogen deploys iminosugar galactosyrin to manipulate plant glycobiology.","description":"The extracellular space (apoplast) of plants is an important molecular battleground during infection by many pathogens. We previously found that a plant-secreted β-galactosidase BGAL1 acts in immunity by facilitating the release of immunogenic peptides from bacterial flagellin and that <i>Pseudomonas syringae</i> suppresses this enzyme by producing a small molecule inhibitor called galactosyrin. Here, we elucidated the structure and biosynthesis of galactosyrin and uncovered its multifunctional roles during infection. Structural elucidation by cryo-EM and chemical synthesis revealed that galactosyrin is an iminosugar featuring a unique geminal diol attached to the pyrrolidine moiety that mimics galactose binding to the β-galactosidase active site. Galactosyrin biosynthesis branches off fro","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-05-26T14:03:23.78Z","creation":"2025-04-04T13:11:01.876Z"},"accession":"S-EPMC11844564","cross_references":{"pubmed":["39990308"],"doi":["10.1101/2025.02.13.638044"]}}