biostudies-literatureUnknown31Chen LPatients with diabetic foot ulcers (DFU) suffering from severe lower limb ischemia face the risk of amputation. Concomitant oxidative stress and hyperinflammation commonly manifest within the tissue affected by DFU, exacerbating the deterioration of DFU wounds. One-two punch strategy of anti-oxidative damage plus anti-inflammatory is anticipated to tackle the challenge of non-healing diabetic wounds. Here, we introduced a dual-approach treatment strategy involving the probiotic <i>Weissella cibaria</i> (WC) modified with desferrioxamine (DFO). This engineered probiotic, known as WC@DPA, aims to ameliorate oxidative stress within the ischemic microenvironment and stimulate the formation and proliferation of endothelial tubular structures. When applied with chronic wounds and ischemic hindliMaterials today. Bio101548https://www.ebi.ac.uk/biostudies/studies/S-EPMC11847733biostudies-literatureA one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers.PMC11847733Li YMa LChen LZhang XChen HZhang CfalseA one-two punch of inflammation and oxidative stress promotes revascularization for diabetic foot ulcers.Patients with diabetic foot ulcers (DFU) suffering from severe lower limb ischemia face the risk of amputation. Concomitant oxidative stress and hyperinflammation commonly manifest within the tissue affected by DFU, exacerbating the deterioration of DFU wounds. One-two punch strategy of anti-oxidative damage plus anti-inflammatory is anticipated to tackle the challenge of non-healing diabetic wounds. Here, we introduced a dual-approach treatment strategy involving the probiotic <i>Weissella cibaria</i> (WC) modified with desferrioxamine (DFO). This engineered probiotic, known as WC@DPA, aims to ameliorate oxidative stress within the ischemic microenvironment and stimulate the formation and proliferation of endothelial tubular structures. When applied with chronic wounds and ischemic hindli2025-01-01T00:00:00Z2025 Apr2025-04-04T09:09:56.402Z2025-04-04T09:09:56.402ZS-EPMC118477333999557810.1016/j.mtbio.2025.101548