biostudies-literatureUnknown11(3)Osman AA<h4>Background and objective</h4>Obesity is intricately linked with metabolic disturbances. The comprehensive exploration of metabolomes is important in unravelling the complexities of obesity development. This study was aimed to discern unique metabolite signatures in obese and lean individuals using liquid chromatography-mass spectrometry quadruple time-of-flight (LC-MS/Q-TOF), with the goal of elucidating their roles in obesity.<h4>Methods</h4>A total of 160 serum samples (Discovery, n = 60 and Validation, n = 100) of obese and lean individuals with stable Body Mass Index (BMI) values were retrieved from The Malaysian Cohort biobank. Metabolic profiles were obtained using LC-MS/Q-TOF in dual-polarity mode. Metabolites were identified using a molecular feature and chemical formula algoriHeliyone42197https://www.ebi.ac.uk/biostudies/studies/S-EPMC11848079biostudies-literatureLipids as key biomarkers in unravelling the pathophysiology of obesity-related metabolic dysregulation.PMC11848079Abdul Murad NAJamal RAbdullah NOsman AAChin SFTeh LKfalseLipids as key biomarkers in unravelling the pathophysiology of obesity-related metabolic dysregulation.<h4>Background and objective</h4>Obesity is intricately linked with metabolic disturbances. The comprehensive exploration of metabolomes is important in unravelling the complexities of obesity development. This study was aimed to discern unique metabolite signatures in obese and lean individuals using liquid chromatography-mass spectrometry quadruple time-of-flight (LC-MS/Q-TOF), with the goal of elucidating their roles in obesity.<h4>Methods</h4>A total of 160 serum samples (Discovery, n = 60 and Validation, n = 100) of obese and lean individuals with stable Body Mass Index (BMI) values were retrieved from The Malaysian Cohort biobank. Metabolic profiles were obtained using LC-MS/Q-TOF in dual-polarity mode. Metabolites were identified using a molecular feature and chemical formula algori2025-01-01T00:00:00Z2025 Feb2025-04-03T23:44:17.253Z2025-04-03T23:44:17.253ZS-EPMC118480793999592310.1016/j.heliyon.2025.e42197