biostudies-literatureUnknown11(3)Yaikhan T<i>Limosilactobacillus fermentum</i> has gained recognition as a probiotic due to its immunomodulatory properties. In this study, we characterized <i>L. fermentum</i> SD7, which was isolated from the human oral cavity. The genome of <i>L. fermentum</i> SD7 was approximately 2.27 Mb in size, with a 51.1 % GC content. Using comprehensive genome analysis, we compared the genome of <i>L. fermentum</i> SD7 with 153 available genome sequences of <i>L. fermentum</i> strains and categorized the 154 strains into six distinct clades based on core gene single nucleotide polymorphisms. Among the 12,598 orthologous proteins, we identified 910 core genes and 10,169 accessory genes. Our analysis revealed a close similarity between <i>L. fermentum</i> SD7, FS-10, and L13. In addition, <i>L. fermentum</i> Heliyone42451https://www.ebi.ac.uk/biostudies/studies/S-EPMC11850171biostudies-literatureProbiogenomic analysis of <i>Limosilactobacillus fermentum</i> SD7, a probiotic candidate with remarkable aggregation abilities.PMC11850171Wonglapsuwan MPahumunto NNokchan NYaikhan TTeanpaisan RSurachat KfalseProbiogenomic analysis of <i>Limosilactobacillus fermentum</i> SD7, a probiotic candidate with remarkable aggregation abilities.<i>Limosilactobacillus fermentum</i> has gained recognition as a probiotic due to its immunomodulatory properties. In this study, we characterized <i>L. fermentum</i> SD7, which was isolated from the human oral cavity. The genome of <i>L. fermentum</i> SD7 was approximately 2.27 Mb in size, with a 51.1 % GC content. Using comprehensive genome analysis, we compared the genome of <i>L. fermentum</i> SD7 with 153 available genome sequences of <i>L. fermentum</i> strains and categorized the 154 strains into six distinct clades based on core gene single nucleotide polymorphisms. Among the 12,598 orthologous proteins, we identified 910 core genes and 10,169 accessory genes. Our analysis revealed a close similarity between <i>L. fermentum</i> SD7, FS-10, and L13. In addition, <i>L. fermentum</i> 2025-01-01T00:00:00Z2025 Feb2026-06-02T08:09:01.549Z2025-04-04T13:46:29.463ZS-EPMC118501714000777210.1016/j.heliyon.2025.e42451