biostudies-literatureYou ZHeilongjiang Postdoctoral GrantChina Postdoctoral Science FoundationNational Natural Science Foundation of China77https://www.ebi.ac.uk/biostudies/studies/S-EPMC11852879biostudies-literatureUnknown31(1)<h4>Background</h4>The pathogenesis of epilepsy is complex, and current antiepileptic drugs do not effectively control the seizures. Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) regulates neuronal excitability, but its mechanism of action in epilepsy is not clear. In this paper, we investigated the effect of CPEB3 on seizures and elucidated its underlying molecular mechanism.<h4>Methods</h4>Bioinformatics-based search for genes closely associated with epilepsy. Changes in expression and cellular localization of CPEB3 in epilepsy were verified by western blotting (WB) and Immunofluorescence staining. Subsequently, The adeno-associated virus was employed to overexpress or knockdown in mice. Behavioral experiments verified the effect of CPEB3 on epileptic phenotype, and the mMolecular medicine (Cambridge, Mass.)CPEB3 can regulate seizure susceptibility by inhibiting the transcriptional activity of STAT3 on NMDARs expression.PMC11852879LBH-Q20045LBH-Q211322018M64030681871016Liu XLiu YHuang CYou ZLi JFan ZWei FGao XSun JfalseCPEB3 can regulate seizure susceptibility by inhibiting the transcriptional activity of STAT3 on NMDARs expression.<h4>Background</h4>The pathogenesis of epilepsy is complex, and current antiepileptic drugs do not effectively control the seizures. Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) regulates neuronal excitability, but its mechanism of action in epilepsy is not clear. In this paper, we investigated the effect of CPEB3 on seizures and elucidated its underlying molecular mechanism.<h4>Methods</h4>Bioinformatics-based search for genes closely associated with epilepsy. Changes in expression and cellular localization of CPEB3 in epilepsy were verified by western blotting (WB) and Immunofluorescence staining. Subsequently, The adeno-associated virus was employed to overexpress or knockdown in mice. Behavioral experiments verified the effect of CPEB3 on epileptic phenotype, and the m2025-01-01T00:00:00Z2025 Feb2025-04-18T13:06:05.552Z2025-04-06T22:36:18.449ZS-EPMC118528793999458710.1186/s10020-025-01136-2