biostudies-literatureLopez-Ruiz MDivisión de Investigación Bogotá (DIB)-Universidad Nacional de ColombiaWellcome Trust439https://www.ebi.ac.uk/biostudies/studies/S-EPMC11853030biostudies-literatureUnknown13(2)<b>Background/Objective</b>: Finding new targets to attenuate <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) is key in the development of new TB vaccines. In this context, plasma membrane P-type ATPases are relevant for mycobacterial homeostasis and virulence. In this work, we investigate the role of the copper-transporting P-type ATPase CtpA in <i>Mtb</i> virulence. <b>Methods</b>: The impact of CtpA deletion on <i>Mtb</i>'s capacity to overcome redox stress and proliferate in mouse alveolar macrophages (MH-S) was evaluated, as well as its effect on <i>Mtb</i> immunogenicity. Moreover, the influence of CtpA on the pathogenicity of <i>Mtb</i> in a mouse (BALB/c) model of progressive TB was examined. <b>Results</b>: We found that MH-S cells infected with wild-type (<i>Mtb</i>H37Rv) or the mBiomedicinesThe Plasma Membrane P-Type ATPase CtpA Is Required for <i>Mycobacterium tuberculosis</i> Virulence in Copper-Activated Macrophages in a Mouse Model of Progressive Tuberculosis.PMC1185303047274, 50299, 57532 and 60311223279Lopez-Ruiz MMaya-Hoyos MBarrios-Payan JHernandez-Pando RMata-Espinosa DSoto CYOcampo MfalseThe Plasma Membrane P-Type ATPase CtpA Is Required for <i>Mycobacterium tuberculosis</i> Virulence in Copper-Activated Macrophages in a Mouse Model of Progressive Tuberculosis.<b>Background/Objective</b>: Finding new targets to attenuate <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) is key in the development of new TB vaccines. In this context, plasma membrane P-type ATPases are relevant for mycobacterial homeostasis and virulence. In this work, we investigate the role of the copper-transporting P-type ATPase CtpA in <i>Mtb</i> virulence. <b>Methods</b>: The impact of CtpA deletion on <i>Mtb</i>'s capacity to overcome redox stress and proliferate in mouse alveolar macrophages (MH-S) was evaluated, as well as its effect on <i>Mtb</i> immunogenicity. Moreover, the influence of CtpA on the pathogenicity of <i>Mtb</i> in a mouse (BALB/c) model of progressive TB was examined. <b>Results</b>: We found that MH-S cells infected with wild-type (<i>Mtb</i>H37Rv) or the m2025-01-01T00:00:00Z2025 Feb2025-04-04T10:41:43.279Z2025-04-04T10:41:43.279ZS-EPMC118530304000285210.3390/biomedicines13020439