biostudies-literatureHou SMNational Science and Technology Council, TaiwanShin Kong Wu Ho-Su Memorial HospitalChi Mei Medical Center-Taipei Medical UniversityCathay General Hospital174https://www.ebi.ac.uk/biostudies/studies/S-EPMC11853366biostudies-literatureUnknown15(2)Inflammation, a fundamental response to infection and injury, involves interactions among immune cells and signaling molecules. Dysregulated inflammation contributes to diseases such as autoimmune disorders and cancer. Interleukin-1 beta (IL-1β), produced by macrophages in response to lipoteichoic acid (LTA) from Gram-positive bacteria, is a key inflammatory mediator. Glabridin (GBD), a bioactive compound from licorice root, exhibits anti-inflammatory properties. This study investigates GBD's effects on LTA-induced proinflammatory signaling in RAW 264.7 murine macrophages and alveolar macrophages, MH-S, focusing on <i>IL-1β</i> expression and signaling pathways. Cell viability assays confirmed that 20 μM GBD was non-cytotoxic. Confocal microscopy and quantitative PCR showed that GBD signifBiomoleculesGlabridin Suppresses Macrophage Activation by Lipoteichoic Acid In Vitro: The Crucial Role of MAPKs-IL-1β-iNOS Axis Signals in Peritoneal and Alveolar Macrophages.PMC118533662023SKHAND006MOST 110-2320-B341-001-MY3NSTC 113-2320-B341-002-MY3110CM-TMU-18CGH-MR-A112262024SKHAND008Hsia CHHou SMHsia CWCheng SWYang CMYen TLHsieh CYHuang WCfalseGlabridin Suppresses Macrophage Activation by Lipoteichoic Acid In Vitro: The Crucial Role of MAPKs-IL-1β-iNOS Axis Signals in Peritoneal and Alveolar Macrophages.Inflammation, a fundamental response to infection and injury, involves interactions among immune cells and signaling molecules. Dysregulated inflammation contributes to diseases such as autoimmune disorders and cancer. Interleukin-1 beta (IL-1β), produced by macrophages in response to lipoteichoic acid (LTA) from Gram-positive bacteria, is a key inflammatory mediator. Glabridin (GBD), a bioactive compound from licorice root, exhibits anti-inflammatory properties. This study investigates GBD's effects on LTA-induced proinflammatory signaling in RAW 264.7 murine macrophages and alveolar macrophages, MH-S, focusing on <i>IL-1β</i> expression and signaling pathways. Cell viability assays confirmed that 20 μM GBD was non-cytotoxic. Confocal microscopy and quantitative PCR showed that GBD signif2025-01-01T00:00:00Z2025 Jan2025-04-22T20:07:18.333Z2025-04-06T03:01:47.528ZS-EPMC118533664000147710.3390/biom15020174