biostudies-literatureYan ZZhen QinHaiyan Gao662https://www.ebi.ac.uk/biostudies/studies/S-EPMC11853853biostudies-literatureUnknown14(4)<i>Staphylococcus aureus</i> (<i>S. aureus</i>) can contaminate food by forming biofilms, leading to significant food safety concerns. <i>Amomum tsaoko</i> essential oil (AEO) has been shown to be an effective plant-derived antibacterial agent. This study investigated the antibiofilm activity of AEO and evaluated its potential benefit in pork preservation. The results showed that AEO solution (2 mg/mL) can effectively remove the biofilm of <i>S. aureus</i> on food contact materials, achieving a removal rate of over 90%. Scanning electron microscopy (SEM) revealed that the <i>S. aureus</i> biofilm structure was disrupted after treatment with AEO. Meanwhile, AEO treatment significantly reduced the initial formation of <i>S. aureus</i> biofilms and extracellular polymeric substance (EPS) prodFoods (Basel, Switzerland)Antibiofilm Activity of <i>Amomum tsaoko</i> Essential Oil on <i>Staphylococcus aureus</i> and Its Application in Pork Preservation.PMC118538533217216032272390Wan SChen QYan ZGuo JQin ZGao HfalseAntibiofilm Activity of <i>Amomum tsaoko</i> Essential Oil on <i>Staphylococcus aureus</i> and Its Application in Pork Preservation.<i>Staphylococcus aureus</i> (<i>S. aureus</i>) can contaminate food by forming biofilms, leading to significant food safety concerns. <i>Amomum tsaoko</i> essential oil (AEO) has been shown to be an effective plant-derived antibacterial agent. This study investigated the antibiofilm activity of AEO and evaluated its potential benefit in pork preservation. The results showed that AEO solution (2 mg/mL) can effectively remove the biofilm of <i>S. aureus</i> on food contact materials, achieving a removal rate of over 90%. Scanning electron microscopy (SEM) revealed that the <i>S. aureus</i> biofilm structure was disrupted after treatment with AEO. Meanwhile, AEO treatment significantly reduced the initial formation of <i>S. aureus</i> biofilms and extracellular polymeric substance (EPS) prod2025-01-01T00:00:00Z2025 Feb2025-04-03T23:35:58.628Z2025-04-03T23:35:58.628ZS-EPMC118538534000210810.3390/foods14040662