biostudies-literatureChuang TDNICHD NIH HHSNIH HHS1688https://www.ebi.ac.uk/biostudies/studies/S-EPMC11854932biostudies-literatureUnknown26(4)We performed next-generation sequencing (NGS) on RNA from 19 paired leiomyoma (Lyo) and myometrium (Myo) specimens, stratified by race/ethnicity (White: n = 7; Black: n = 12) and mediator complex subunit 12 (MED12) mutation status (mutated: n = 10; non-mutated: n = 9). Analysis identified 2,189 small non-coding RNAs (sncRNAs) with altered expression in Lyo compared to paired Myo (≥1.5-fold change), including small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), microRNAs (miRNAs), and PIWI-interacting RNAs (piRNAs). Among these, 17 sncRNAs showed differential expression in the MED12-mutated group versus Myo, while minimal changes were observed in the non-mutated group. Additionally, 31 sncRNAs displayed differential expression in Black women compared to White women. For validation, International journal of molecular sciencesDifferential Expression of Small Non-Coding RNAs in Uterine Leiomyomas.PMC11854932R01 HD109286HD100529R03 HD101852HD109286HD101852R01 HD100529Ton NBaghdasarian DChuang TDRysling SKhorram OfalseDifferential Expression of Small Non-Coding RNAs in Uterine Leiomyomas.We performed next-generation sequencing (NGS) on RNA from 19 paired leiomyoma (Lyo) and myometrium (Myo) specimens, stratified by race/ethnicity (White: n = 7; Black: n = 12) and mediator complex subunit 12 (MED12) mutation status (mutated: n = 10; non-mutated: n = 9). Analysis identified 2,189 small non-coding RNAs (sncRNAs) with altered expression in Lyo compared to paired Myo (≥1.5-fold change), including small nuclear RNAs (snRNAs), small nucleolar RNAs (snoRNAs), microRNAs (miRNAs), and PIWI-interacting RNAs (piRNAs). Among these, 17 sncRNAs showed differential expression in the MED12-mutated group versus Myo, while minimal changes were observed in the non-mutated group. Additionally, 31 sncRNAs displayed differential expression in Black women compared to White women. For validation, 2025-01-01T00:00:00Z2025 Feb2026-05-26T20:12:41.988Z2025-04-07T07:47:02.478ZS-EPMC118549324000415210.3390/ijms26041688