biostudies-literatureLiu SNational Natural Science Foundation of ChinaNatural Science Basic Research Program of Shaanxi Province1472https://www.ebi.ac.uk/biostudies/studies/S-EPMC11855083biostudies-literatureUnknown26(4)The type VI secretion system (T6SS) is a widely distributed molecular apparatus found in most Gram-negative bacteria. Studies show that T6SSs have functions in bacterial virulence, inter- and intra-bacterial competition, and environmental adaptation. <i>Pseudomonas aeruginosa</i>, an opportunistic pathogen, harbors three T6SS gene clusters that perform diverse roles in clinical infection. Herein, using DNA affinity chromatography of the H1-T6SS promoter, the fluoroquinolone antibiotic resistance regulator NfxB was identified. Further studies demonstrated that NfxB negatively regulates the expression of H1-T6SS by directly binding to its promoter region. T6SS expression and effector secretion are regulated by the fluoroquinolone antibiotic via NfxB, which enhances inter-bacterial competitioInternational journal of molecular sciencesRegulation of the H1 Type VI Secretion System by the Transcriptional Regulator NfxB in <i>Pseudomonas aeruginosa</i>.PMC11855083322701952022JQ-184 and 2024JC-YBQN-0176Cao LLiu LLiu QLiu SYan WZhao YLi MWu ZYang YZhang YWang TTao RGao TfalseRegulation of the H1 Type VI Secretion System by the Transcriptional Regulator NfxB in <i>Pseudomonas aeruginosa</i>.The type VI secretion system (T6SS) is a widely distributed molecular apparatus found in most Gram-negative bacteria. Studies show that T6SSs have functions in bacterial virulence, inter- and intra-bacterial competition, and environmental adaptation. <i>Pseudomonas aeruginosa</i>, an opportunistic pathogen, harbors three T6SS gene clusters that perform diverse roles in clinical infection. Herein, using DNA affinity chromatography of the H1-T6SS promoter, the fluoroquinolone antibiotic resistance regulator NfxB was identified. Further studies demonstrated that NfxB negatively regulates the expression of H1-T6SS by directly binding to its promoter region. T6SS expression and effector secretion are regulated by the fluoroquinolone antibiotic via NfxB, which enhances inter-bacterial competitio2025-01-01T00:00:00Z2025 Feb2025-04-21T17:09:45.319Z2025-04-21T17:09:45.319ZS-EPMC118550834000393710.3390/ijms26041472