{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(2)"],"submitter":["Hong W"],"pubmed_abstract":["Skeletal muscle exhibits remarkable plasticity in response to diverse stimuli, with exercise serving as a potent trigger. Varied exercise modalities, including moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT), induce distinct structural and functional adaptations on skeletal muscle. However, the underlying molecular mechanisms governing these adaptations remain poorly understood. In this study, we utilized RNA-seq to characterize the transcriptomic profile of murine gastrocnemius muscle following 8-week treadmill-based MICT (M group) and HIIT (H group). A total of 1052 DEGs were screened in H vs. M. Among the top 10 significant DEGs, Foxo1 and Myod1 are closely related to muscular physiology. Through KEGG pathway analysis, distinct adaptations were primarily identified in the FoxO, MAPK, and PI3K-AKT pathways. By analyzing the expression of myokines, a significantly higher Igf-1 expression level was observed in the M group compared to the H group. Therefore, IGF-1, a well-known upstream regulator of both the PI3K-AKT-FoxO and MAPK pathways, might drive distinct muscle adaptations through variations in Igf-1 expression induced by these two exercise modalities."],"journal":["PloS one"],"pagination":["e0318782"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11856427"],"repository":["biostudies-literature"],"pubmed_title":["Transcriptomic adaptation of skeletal muscle in response to MICT and HIIT exercise modalities."],"pmcid":["PMC11856427"],"pubmed_authors":["Xiong Y","Luan Y","Zheng J","Ma Y","Hong W","Zhang B"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic adaptation of skeletal muscle in response to MICT and HIIT exercise modalities.","description":"Skeletal muscle exhibits remarkable plasticity in response to diverse stimuli, with exercise serving as a potent trigger. Varied exercise modalities, including moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT), induce distinct structural and functional adaptations on skeletal muscle. However, the underlying molecular mechanisms governing these adaptations remain poorly understood. In this study, we utilized RNA-seq to characterize the transcriptomic profile of murine gastrocnemius muscle following 8-week treadmill-based MICT (M group) and HIIT (H group). A total of 1052 DEGs were screened in H vs. M. Among the top 10 significant DEGs, Foxo1 and Myod1 are closely related to muscular physiology. Through KEGG pathway analysis, distinct adaptations were primarily identified in the FoxO, MAPK, and PI3K-AKT pathways. By analyzing the expression of myokines, a significantly higher Igf-1 expression level was observed in the M group compared to the H group. Therefore, IGF-1, a well-known upstream regulator of both the PI3K-AKT-FoxO and MAPK pathways, might drive distinct muscle adaptations through variations in Igf-1 expression induced by these two exercise modalities.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2025-04-03T23:44:20.448Z","creation":"2025-04-03T23:44:20.448Z"},"accession":"S-EPMC11856427","cross_references":{"pubmed":["39999092"],"doi":["10.1371/journal.pone.0318782"]}}