biostudies-literatureKomissarov ENgovernment assignment for FRC Kazan Scientific Center of RAS140https://www.ebi.ac.uk/biostudies/studies/S-EPMC11856433biostudies-literatureUnknown11(2)The host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>), normally distributed on the accessory chromosomes. The pattern of <i>mimp</i> associated with effector genes has been used to predict candidate effector profiles which characterize <i>Fox</i> formae speciales. In this study, we demonstrate the pathogenicity of strains <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407 and <i>Fusarium oxysporum</i> f.sp. <i>radicis-cucumerinum</i> (<i>Forc</i>) V03-2g in a common host plant (cucumber) and compare their genomes. The <i>Forl</i> ZUM2407 genome lacks <i>SIX</i> genes and their homologs, in contrast to <i>Forc</i> V03-2g. We predicted the total number of <i>mimp</i> elements in the genome of <i>Forl</i> ZUM2407 to be three-fold less than that of <i>Forc</i> V03-2g (10 and 36 copies, respectively). The <i>mimp</i> distribution pattern in <i>Forl</i> ZUM2407 was completely different from that present in <i>Forc</i> V03-2g. Candidate effector profile analysis did not predict that <i>Forl</i> ZUM2407 was able to infect cucumber plants like <i>Forc</i> V03-2g. Therefore, we assume that <i>Forl</i> ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the <i>mimp</i>-based approach.Journal of fungi (Basel, Switzerland)Genomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber.PMC11856433124050300050-4Dvorianinova EMBruskin SADmitriev AAKomissarov ENAbdeeva IAfordoanyi DMValidov SZGudkov SVDiabankana RGCfalseGenomic Differences Between Two <i>Fusarium oxysporum</i> Formae Speciales Causing Root Rot in Cucumber.The host specificity of <i>Fusarium oxysporum</i> (<i>Fox</i>) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (<i>mimp</i>), normally distributed on the accessory chromosomes. The pattern of <i>mimp</i> associated with effector genes has been used to predict candidate effector profiles which characterize <i>Fox</i> formae speciales. In this study, we demonstrate the pathogenicity of strains <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407 and <i>Fusarium oxysporum</i> f.sp. <i>radicis-cucumerinum</i> (<i>Forc</i>) V03-2g in a common host plant (cucumber) and compare their genomes. The <i>Forl</i> ZUM2407 genome lacks <i>SIX</i> genes and their homologs, in contrast to <i>Forc</i> V03-2g. We predicted the total number of <i>mimp</i> elements in the genome of <i>Forl</i> ZUM2407 to be three-fold less than that of <i>Forc</i> V03-2g (10 and 36 copies, respectively). The <i>mimp</i> distribution pattern in <i>Forl</i> ZUM2407 was completely different from that present in <i>Forc</i> V03-2g. Candidate effector profile analysis did not predict that <i>Forl</i> ZUM2407 was able to infect cucumber plants like <i>Forc</i> V03-2g. Therefore, we assume that <i>Forl</i> ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the <i>mimp</i>-based approach.2025-01-01T00:00:00Z2025 Feb2026-04-08T19:48:21.29Z2025-04-19T21:19:27.358ZS-EPMC118564333999743410.3390/jof11020140