biostudies-literatureSankhyan SSharda University31https://www.ebi.ac.uk/biostudies/studies/S-EPMC11856512biostudies-literatureUnknown15(1)Polyaromatic hydrocarbons (PAHs) are a group of organic pollutants commonly found in the environment due to industrial activities, incomplete burning of fossil fuels, and oil spills. Bioremediation of PAHs has emerged as a promising approach. This study investigated the biodegradation of PAHs (anthracene, naphthalene, phenanthrene, anthraquinone, and anthrone) at 100 ppm to 1000 ppm in the presence of glucose and glycerol by a biosurfactant-producing strain of <i>Pseudomonas aeruginosa</i> NG4. The quality of the biosurfactant produced by the bacterial strain was analyzed via emulsion index (E24), drop-collapse assay, and oil displacement assay. The PAH degradation efficiency was studied by HPLC and degradation metabolites were analyzed using GC-MS. Among all five PAHs (fed at 300 ppm), thJournal of xenobioticsDegradation of Polyaromatic Hydrocarbons by Biosurfactant-Producing <i>Pseudomonas aeruginosa</i> NG4.PMC11856512SU/SF/2022/04Sankhyan SPandit SKumar PSharma KRay SfalseDegradation of Polyaromatic Hydrocarbons by Biosurfactant-Producing <i>Pseudomonas aeruginosa</i> NG4.Polyaromatic hydrocarbons (PAHs) are a group of organic pollutants commonly found in the environment due to industrial activities, incomplete burning of fossil fuels, and oil spills. Bioremediation of PAHs has emerged as a promising approach. This study investigated the biodegradation of PAHs (anthracene, naphthalene, phenanthrene, anthraquinone, and anthrone) at 100 ppm to 1000 ppm in the presence of glucose and glycerol by a biosurfactant-producing strain of <i>Pseudomonas aeruginosa</i> NG4. The quality of the biosurfactant produced by the bacterial strain was analyzed via emulsion index (E24), drop-collapse assay, and oil displacement assay. The PAH degradation efficiency was studied by HPLC and degradation metabolites were analyzed using GC-MS. Among all five PAHs (fed at 300 ppm), th2025-01-01T00:00:00Z2025 Feb2026-05-24T03:15:21.268Z2025-04-04T02:54:14.483ZS-EPMC118565123999737410.3390/jox15010031