biostudies-literatureSun YNIAID NIH HHS1542531https://www.ebi.ac.uk/biostudies/studies/S-EPMC11868092biostudies-literatureUnknown16<h4>Introduction</h4>Cytomegalovirus (CMV) viremia remains a major contributor to clinical complications in solid organ transplant (SOT) patients, including organ injury, morbidity and mortality. Given their critical role in antiviral defense, CD8+ T cells are essential for protective immunity against CMV.<h4>Methods</h4>Using single-cell RNA sequencing, we investigated the transcriptional signatures and developmental lineages of CD8+ T cells in eight immunosuppressed kidney transplant recipients (KTRs) who received organs from CMV-seropositive donors. Results were validated in a cohort of 62 KTRs using immunophenotyping.<h4>Results</h4>Our data revealed a significant influence of CMV serostatus on transcriptional variance of CD8+ memory T cells, associating with the first principal componFrontiers in immunologyCytotoxic KLRG1+ IL-7R- effector CD8+ T cells distinguish kidney transplant recipients controlling cytomegalovirus reactivation.PMC11868092U19 AI128913Schaenman JMSarwal MMLanier LLCappelletti MArakawa-Hoyt JSun YSen SSigdel TKRossetti MBunnapradist SPickering HReed EFGjertson DWParmar RfalseCytotoxic KLRG1+ IL-7R- effector CD8+ T cells distinguish kidney transplant recipients controlling cytomegalovirus reactivation.<h4>Introduction</h4>Cytomegalovirus (CMV) viremia remains a major contributor to clinical complications in solid organ transplant (SOT) patients, including organ injury, morbidity and mortality. Given their critical role in antiviral defense, CD8+ T cells are essential for protective immunity against CMV.<h4>Methods</h4>Using single-cell RNA sequencing, we investigated the transcriptional signatures and developmental lineages of CD8+ T cells in eight immunosuppressed kidney transplant recipients (KTRs) who received organs from CMV-seropositive donors. Results were validated in a cohort of 62 KTRs using immunophenotyping.<h4>Results</h4>Our data revealed a significant influence of CMV serostatus on transcriptional variance of CD8+ memory T cells, associating with the first principal compon2025-01-01T00:00:00Z20252025-04-03T23:36:46.17Z2025-04-03T23:36:46.17ZS-EPMC118680924002834210.3389/fimmu.2025.1542531