{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tsai YX"],"funding":["Academia Sinica"],"pagination":["2045"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11868633"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Human coronavirus 229E (HCoV-229E) is the earliest CoV found to infect humans. It binds to the human aminopeptidase N (hAPN) through the receptor binding domain (RBD) of its spike (S) protein to achieve host recognition. We present the cryo-electron microscopy structure of two HCoV-229E S protein in complex with a dimeric hAPN to provide structural insights on how the HCoV-229E S protein opens up its RBD to engage with its host receptor, information that is currently missing among alphacoronaviruses to which HCoV-229E belong. We quantitatively profile the glycosylation of HCoV-229E S protein and hAPN to deduce the glyco-shielding effects pertinent to antigenicity and host recognition. Finally, we present an atomic model of fully glycosylated HCoV-229E S in complex with hAPN anchored on the"],"journal":["Nature communications"],"pubmed_title":["Molecular basis of host recognition of human coronavirus 229E."],"pmcid":["PMC11868633"],"funding_grant_id":["AS-IV-114-L04","AS-IDR-114-S01","AS-IDR-111-03","AS-IDR-112-04"],"pubmed_authors":["Hsu SD","Tsai YX","Chien YC","Hsu MF","Khoo KH"],"additional_accession":[]},"is_claimable":false,"name":"Molecular basis of host recognition of human coronavirus 229E.","description":"Human coronavirus 229E (HCoV-229E) is the earliest CoV found to infect humans. It binds to the human aminopeptidase N (hAPN) through the receptor binding domain (RBD) of its spike (S) protein to achieve host recognition. We present the cryo-electron microscopy structure of two HCoV-229E S protein in complex with a dimeric hAPN to provide structural insights on how the HCoV-229E S protein opens up its RBD to engage with its host receptor, information that is currently missing among alphacoronaviruses to which HCoV-229E belong. We quantitatively profile the glycosylation of HCoV-229E S protein and hAPN to deduce the glyco-shielding effects pertinent to antigenicity and host recognition. Finally, we present an atomic model of fully glycosylated HCoV-229E S in complex with hAPN anchored on the","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-06-02T19:14:19.033Z","creation":"2025-04-20T01:46:03.112Z"},"accession":"S-EPMC11868633","cross_references":{"pubmed":["40016196"],"doi":["10.1038/s41467-025-57359-8"]}}