biostudies-literatureChen YShanghai Education Development FoundationShanghai Municipal Education CommissionNatural Science Foundation of Shanghai MunicipalityNational Natural Science Foundation of ChinaState Key Laboratory of Bioreactor EngineeringNational Key Research and Development Program of China4716-4724https://www.ebi.ac.uk/biostudies/studies/S-EPMC11869998biostudies-literatureUnknown73(8)Itaconic acid (IA) is a multifunctional platform chemical with numerous biological functions. Here, <i>Trichoderma reesei</i> was engineered as a remarkable cell factory to produce IA. Heterologous overexpression of the mitochondrial tricarboxylate transport protein and <i>cis</i>-aconitate decarboxylase from <i>Aspergillus terreus</i> in <i>T. reesei</i> initiated IA production with a titer of 20 g/L. By increasing the copy number of <i>mttA</i> and <i>cadA</i> and the overexpression of the plasma membrane transporter proteins (MFSA), the titer of IA reached 56.7 g/L. The precursor synthesis pathway of IA was overexpressed by the overexpression of aconitase and citrate synthase, and the IA competition pathway was blocked by the deletion of the P450 monooxygenase gene <i>cyp3</i> to furtheJournal of agricultural and food chemistryMetabolic Engineering of Filamentous Fungus <i>Trichoderma reesei</i> for Itaconic Acid Production.PMC11869998323700932022YFA0912300202222ZR141760021CGA3423ZR1414800Lv JLi XLiu JWei DChen YWang WChen CWang ZXiao SLin LfalseMetabolic Engineering of Filamentous Fungus <i>Trichoderma reesei</i> for Itaconic Acid Production.Itaconic acid (IA) is a multifunctional platform chemical with numerous biological functions. Here, <i>Trichoderma reesei</i> was engineered as a remarkable cell factory to produce IA. Heterologous overexpression of the mitochondrial tricarboxylate transport protein and <i>cis</i>-aconitate decarboxylase from <i>Aspergillus terreus</i> in <i>T. reesei</i> initiated IA production with a titer of 20 g/L. By increasing the copy number of <i>mttA</i> and <i>cadA</i> and the overexpression of the plasma membrane transporter proteins (MFSA), the titer of IA reached 56.7 g/L. The precursor synthesis pathway of IA was overexpressed by the overexpression of aconitase and citrate synthase, and the IA competition pathway was blocked by the deletion of the P450 monooxygenase gene <i>cyp3</i> to furthe2025-01-01T00:00:00Z2025 Feb2026-07-16T00:37:56.111Z2026-07-09T10:26:23.893ZS-EPMC118699983996305110.1021/acs.jafc.4c10107