biostudies-literatureOu SNatural Science Funding of Guangdong ProvinceNational Natural Science Foundation of China599-609https://www.ebi.ac.uk/biostudies/studies/S-EPMC11871191biostudies-literatureUnknown42(2)<h4>Purpose</h4>To elucidatethe epigenetic alteration associated with impaired oogenesis in endometrioma using multi-omic approaches.<h4>Methods</h4>ATAC-seq was performed on the granulosa cells (GCs) of 6 patients (3 with endometrioma and 3 without). Follicular samples from another 20 patients (10 with endometrioma and 10 without) were collected for mRNA-seq analysis of GCs and extracellular vesicles (EVs) of follicular fluid. qRT-PCR validated candidate genes in GCs from 44 newly enrolled patients (19 with endometrioma and 25 without). mRNA abundance was compared with the Mann-Whitney test. Pearson's correlation analyzed relationships between candidate genes and oocyte parameters.<h4>Results</h4>Chromatin accessibility and gene expression profiles of GCs from endometrioma patients differJournal of assisted reproduction and geneticsComparison of chromatin accessibility remodeling of granulosa cells in patients with endometrioma or pelvic/tubal infertility.PMC118711913217061242100150922017A0303138958217164281671523Li YHuang JPan PCao CWang WLi ROu SSun XJiao XZhang QWei LfalseComparison of chromatin accessibility remodeling of granulosa cells in patients with endometrioma or pelvic/tubal infertility.<h4>Purpose</h4>To elucidatethe epigenetic alteration associated with impaired oogenesis in endometrioma using multi-omic approaches.<h4>Methods</h4>ATAC-seq was performed on the granulosa cells (GCs) of 6 patients (3 with endometrioma and 3 without). Follicular samples from another 20 patients (10 with endometrioma and 10 without) were collected for mRNA-seq analysis of GCs and extracellular vesicles (EVs) of follicular fluid. qRT-PCR validated candidate genes in GCs from 44 newly enrolled patients (19 with endometrioma and 25 without). mRNA abundance was compared with the Mann-Whitney test. Pearson's correlation analyzed relationships between candidate genes and oocyte parameters.<h4>Results</h4>Chromatin accessibility and gene expression profiles of GCs from endometrioma patients differ2025-01-01T00:00:00Z2025 Feb2026-06-15T06:27:57.161Z2026-06-15T03:08:44.622ZS-EPMC118711913948557410.1007/s10815-024-03302-7