{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Suchithra KV"],"funding":["Yenepoya University"],"pagination":["7358"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11873149"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the global healthcare concerns. Here, we report the phenotypic and genotypic characterization of a novel multi-host Staphylococcus phage RuSa1, isolated from wastewater samples derived from a spotted sambar deer (Rusa unicolor) enclosure located at Mangalore, India. Clinical MRSA strains (n = 18) susceptible to RuSa1 were genetically and phenotypically diverse as determined by DNA fingerprinting and in vitro culture assays. RuSa1 displayed a latent period and burst size of 10 min and 50 PFU, respectively, and exhibited efficient biofilm removal activities against S. aureus ATCC BAA-44. The phage exhibited moderate UV stability (3 min) and high titre at 4-37 °C and pH 5‒9. RuSa1 possessed a linear double"],"journal":["Scientific reports"],"pubmed_title":["A novel Kayvirus species phage RuSa1 removes biofilm and lyses multiple clinical strains of methicillin resistant Staphylococcus aureus."],"pmcid":["PMC11873149"],"funding_grant_id":["YU/seed grant/127-2022"],"pubmed_authors":["Rekha PD","Arun AB","Stothard P","Suchithra KV","Hameed A"],"additional_accession":[]},"is_claimable":false,"name":"A novel Kayvirus species phage RuSa1 removes biofilm and lyses multiple clinical strains of methicillin resistant Staphylococcus aureus.","description":"The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the global healthcare concerns. Here, we report the phenotypic and genotypic characterization of a novel multi-host Staphylococcus phage RuSa1, isolated from wastewater samples derived from a spotted sambar deer (Rusa unicolor) enclosure located at Mangalore, India. Clinical MRSA strains (n = 18) susceptible to RuSa1 were genetically and phenotypically diverse as determined by DNA fingerprinting and in vitro culture assays. RuSa1 displayed a latent period and burst size of 10 min and 50 PFU, respectively, and exhibited efficient biofilm removal activities against S. aureus ATCC BAA-44. The phage exhibited moderate UV stability (3 min) and high titre at 4-37 °C and pH 5‒9. RuSa1 possessed a linear double","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2025-04-03T23:48:21.839Z","creation":"2025-04-03T23:48:21.839Z"},"accession":"S-EPMC11873149","cross_references":{"pubmed":["40025202"],"doi":["10.1038/s41598-025-92032-6"]}}