biostudies-literatureKawai HJapan Society for the Promotion of Science343-354https://www.ebi.ac.uk/biostudies/studies/S-EPMC11876232biostudies-literatureUnknown486(2)<h4>Introduction</h4>HNF4α expression and SMARCA4 loss were thought to be features of non-terminal respiratory unit (TRU)-type lung adenocarcinomas, but their relationships remained unclear.<h4>Materials and methods</h4>HNF4α-positive cases among 241 lung adenocarcinomas were stratified based on TTF-1 and SMARCA4 expressions, histological subtypes, and driver mutations. Immunohistochemical analysis was performed using xenograft tumors of lung adenocarcinoma cell lines with high HNF4A expression.<h4>Result</h4>HNF4α-positive adenocarcinomas(n = 33) were divided into two groups: the variant group(15 mucinous, 2 enteric, and 1 colloid), where SMARCA4 was retained in all cases, and the conventional non-mucinous group(6 papillary, 5 solid, and 4 acinar), where SMARCA4 was lost in 3/15 cases(20%Virchows Archiv : an international journal of pathologyExpression patterns of HNF4α, TTF-1, and SMARCA4 in lung adenocarcinomas: impacts on clinicopathological and genetic features.PMC1187623222H0284019K0744123K1446918H0263423K0647017K15652Ishikawa SKawai HHagiwara KShiba-Ishii AKawamatsu NFujita KKihara AMiura TAmano YMatsubara DFukayama MShibano TTsubochi HNakagawa TYoshimoto TEndo SSakuma YNiki TUshiku TfalseExpression patterns of HNF4α, TTF-1, and SMARCA4 in lung adenocarcinomas: impacts on clinicopathological and genetic features.<h4>Introduction</h4>HNF4α expression and SMARCA4 loss were thought to be features of non-terminal respiratory unit (TRU)-type lung adenocarcinomas, but their relationships remained unclear.<h4>Materials and methods</h4>HNF4α-positive cases among 241 lung adenocarcinomas were stratified based on TTF-1 and SMARCA4 expressions, histological subtypes, and driver mutations. Immunohistochemical analysis was performed using xenograft tumors of lung adenocarcinoma cell lines with high HNF4A expression.<h4>Result</h4>HNF4α-positive adenocarcinomas(n = 33) were divided into two groups: the variant group(15 mucinous, 2 enteric, and 1 colloid), where SMARCA4 was retained in all cases, and the conventional non-mucinous group(6 papillary, 5 solid, and 4 acinar), where SMARCA4 was lost in 3/15 cases(20%2025-01-01T00:00:00Z2025 Feb2025-04-04T09:10:07.179Z2025-04-04T09:10:07.179ZS-EPMC118762323871094410.1007/s00428-024-03816-6