{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Eneku W"],"funding":["Makerere University","Armed Forces Health Surveillance Branch","US Department of Defense Threat Reduction Agency"],"pagination":["2473159"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11878166"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["<b>Background:</b> Fleas transmit a variety of zoonotic agents whose epidemiology and public health risk remains poorly understood in sub-Saharan Africa, including Uganda particularly outside plague-endemic areas. Common flea-borne zoonotic agents include <i>Rickettsia felis</i> and <i>Yersinia pestis.</i>. <b>Objectives:</b> The study aimed at detecting and characterising flea-borne pathogens in peridomestic environments in Uganda. <b>Methods:</b> We collected fleas from domestic animals, chickens, rodents, and homestead environments; pooled them by species, collection time, and host species. A total of 172 pools were analyzed for <i>Y. pestis Pla</i> gene. Further, 62 pools were tested for <i>Rickettsia</i> species <i>gltA, ompA</i>, and <i>htrA</i> genes by PCR and Sanger sequencing. <b"],"journal":["Infection ecology & epidemiology"],"pubmed_title":["Molecular detection and characterization of &lt;i&gt;Rickettsia felis&lt;/i&gt;, &lt;i&gt;R. asembonensis&lt;/i&gt;, and &lt;i&gt;Yersinia pestis&lt;/i&gt; from peri-domestic fleas in Uganda."],"pmcid":["PMC11878166"],"funding_grant_id":["HDTRA1-15-1-0043"],"pubmed_authors":["Atim G","Erima B","Byarugaba Karuhize D","Mworozi E","Wabwire-Mangen F","Douglas C","Cleary Gillian N","Koehler William J","von Fricken Emery M","Tweyongyere R","Ukuli Aquino Q","Kibuuka H","Eneku W","Tugume T","Byaruhanga Maranda A"],"additional_accession":[]},"is_claimable":false,"name":"Molecular detection and characterization of &lt;i&gt;Rickettsia felis&lt;/i&gt;, &lt;i&gt;R. asembonensis&lt;/i&gt;, and &lt;i&gt;Yersinia pestis&lt;/i&gt; from peri-domestic fleas in Uganda.","description":"<b>Background:</b> Fleas transmit a variety of zoonotic agents whose epidemiology and public health risk remains poorly understood in sub-Saharan Africa, including Uganda particularly outside plague-endemic areas. Common flea-borne zoonotic agents include <i>Rickettsia felis</i> and <i>Yersinia pestis.</i>. <b>Objectives:</b> The study aimed at detecting and characterising flea-borne pathogens in peridomestic environments in Uganda. <b>Methods:</b> We collected fleas from domestic animals, chickens, rodents, and homestead environments; pooled them by species, collection time, and host species. A total of 172 pools were analyzed for <i>Y. pestis Pla</i> gene. Further, 62 pools were tested for <i>Rickettsia</i> species <i>gltA, ompA</i>, and <i>htrA</i> genes by PCR and Sanger sequencing. <b","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-01T21:11:07.004Z","creation":"2025-04-04T02:47:53.787Z"},"accession":"S-EPMC11878166","cross_references":{"pubmed":["40041476"],"doi":["10.1080/20008686.2025.2473159"]}}