{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yalagapati SP"],"funding":["German Research Foundation","WIPANO Wissens und Technologietransfer durch Patente und Nomen"],"pagination":["gkae1242"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11879092"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(4)"],"pubmed_abstract":["In eukaryotes, accurate chromosome segregation during cell division relies on the centromeric histone H3 variant, CENH3. Our previous work identified KINETOCHORE NULL2 (αKNL2) as a plant CENH3 assembly factor, which contains a centromere-targeting motif, CENPC-k, analogous to the CENPC motif found in CENP-C. We also demonstrated that αKNL2 can bind DNA in vitro in a sequence-independent manner, without the involvement of its CENPC-k motif. In this study, we show that the CENPC-k and CENPC motifs alone are insufficient for centromere targeting in Nicotiana benthamiana and Arabidopsis thaliana. In silico analysis identified adjacent DNA-binding regions near the CENPC-k and CENPC motifs, suggesting their role in centromeric DNA interaction. We further demonstrated that protein fragments conta"],"journal":["Nucleic acids research"],"pubmed_title":["Centromeric localization of αKNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions."],"pmcid":["PMC11879092"],"funding_grant_id":["03THWST001","LE2299/5-1","LE2299/3-1"],"pubmed_authors":["Ahmadli U","Berr A","Sinha A","Talbert P","Lermontova I","Yalagapati SP","Dabravolski S","Zuo S","Kalidass M","Rutten T","Yadala R"],"additional_accession":[]},"is_claimable":false,"name":"Centromeric localization of αKNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions.","description":"In eukaryotes, accurate chromosome segregation during cell division relies on the centromeric histone H3 variant, CENH3. Our previous work identified KINETOCHORE NULL2 (αKNL2) as a plant CENH3 assembly factor, which contains a centromere-targeting motif, CENPC-k, analogous to the CENPC motif found in CENP-C. We also demonstrated that αKNL2 can bind DNA in vitro in a sequence-independent manner, without the involvement of its CENPC-k motif. In this study, we show that the CENPC-k and CENPC motifs alone are insufficient for centromere targeting in Nicotiana benthamiana and Arabidopsis thaliana. In silico analysis identified adjacent DNA-binding regions near the CENPC-k and CENPC motifs, suggesting their role in centromeric DNA interaction. We further demonstrated that protein fragments conta","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-05-26T17:02:55.896Z","creation":"2025-04-19T21:15:55.018Z"},"accession":"S-EPMC11879092","cross_references":{"pubmed":["39718987"],"doi":["10.1093/nar/gkae1242"]}}