biostudies-literaturePujadas Liwag EMNIAID NIH HHSNorthwestern UniversityChristina Carinato Charitable FoundationNHGRI NIH HHSNCI NIH HHSNational Institutes of HealthNIGMS NIH HHSNIH HHSNational Science Foundationjcs262161https://www.ebi.ac.uk/biostudies/studies/S-EPMC11883274biostudies-literatureUnknown138(3)Disrupted nuclear shape is associated with multiple pathological processes including premature aging disorders, cancer-relevant chromosomal rearrangements and DNA damage. Nuclear blebs (i.e. herniations of the nuclear envelope) can be induced by (1) nuclear compression, (2) nuclear migration (e.g. cancer metastasis), (3) actin contraction, (4) lamin mutation or depletion, and (5) heterochromatin enzyme inhibition. Recent work has shown that chromatin transformation is a hallmark of bleb formation, but the transformation of higher-order structures in blebs is not well understood. As higher-order chromatin has been shown to assemble into nanoscopic packing domains, we investigated whether (1) packing domain organization is altered within nuclear blebs and (2) whether alteration in packing doJournal of cell scienceNuclear blebs are associated with destabilized chromatin-packing domains.PMC11883274EFMA-1830961U54CA261694T32 AI083216R00 GM123195R35 GM154928UM1 HG0115361UM1HG011536R01 CA228272T32AI083216U54 CA268084R01CA228272R00GM123195U54CA268084EFMA-1830969U54 CA261694Pujadas Liwag EMStephens ADSu YPAlmassalha LMGong RAcosta NBackman VKanemaki MTfalseNuclear blebs are associated with destabilized chromatin-packing domains.Disrupted nuclear shape is associated with multiple pathological processes including premature aging disorders, cancer-relevant chromosomal rearrangements and DNA damage. Nuclear blebs (i.e. herniations of the nuclear envelope) can be induced by (1) nuclear compression, (2) nuclear migration (e.g. cancer metastasis), (3) actin contraction, (4) lamin mutation or depletion, and (5) heterochromatin enzyme inhibition. Recent work has shown that chromatin transformation is a hallmark of bleb formation, but the transformation of higher-order structures in blebs is not well understood. As higher-order chromatin has been shown to assemble into nanoscopic packing domains, we investigated whether (1) packing domain organization is altered within nuclear blebs and (2) whether alteration in packing do2025-01-01T00:00:00Z2025 Feb2026-05-29T14:31:56.79Z2025-04-07T11:26:22.198ZS-EPMC118832743987804510.1242/jcs.262161