biostudies-literatureLiu YNICHD NIH HHSNINDS NIH HHS1113-1126.e6https://www.ebi.ac.uk/biostudies/studies/S-EPMC11890119biostudies-literatureUnknown31(8)Emerging human pluripotent stem cell (hPSC)-based embryo models are useful for studying human embryogenesis. Particularly, there are hPSC-based somitogenesis models using free-floating culture that recapitulate somite formation. Somitogenesis in vivo involves intricately orchestrated biochemical and biomechanical events. However, none of the current somitogenesis models controls biochemical gradients or biomechanical signals in the culture, limiting their applicability to untangle complex biochemical-biomechanical interactions that drive somitogenesis. Herein, we develop a human somitogenesis model by confining hPSC-derived presomitic mesoderm (PSM) tissues in microfabricated trenches. Exogenous microfluidic morphogen gradients imposed on the PSM tissues cause axial patterning and trigger Cell stem cellA human pluripotent stem cell-based somitogenesis model using microfluidics.PMC11890119R01 NS129850R21 NS127983R21 NS113518R21 HD105192R01 HD085121R21 HD109635R21 HD105126Liu YSun SFu JMiao YYan RZYang QKobayashi NKim YSXue XPourquie OfalseA human pluripotent stem cell-based somitogenesis model using microfluidics.Emerging human pluripotent stem cell (hPSC)-based embryo models are useful for studying human embryogenesis. Particularly, there are hPSC-based somitogenesis models using free-floating culture that recapitulate somite formation. Somitogenesis in vivo involves intricately orchestrated biochemical and biomechanical events. However, none of the current somitogenesis models controls biochemical gradients or biomechanical signals in the culture, limiting their applicability to untangle complex biochemical-biomechanical interactions that drive somitogenesis. Herein, we develop a human somitogenesis model by confining hPSC-derived presomitic mesoderm (PSM) tissues in microfabricated trenches. Exogenous microfluidic morphogen gradients imposed on the PSM tissues cause axial patterning and trigger 2024-01-01T00:00:00Z2024 Aug2026-03-16T03:32:49.46Z2025-08-14T03:05:42.139ZS-EPMC118901193898147110.1016/j.stem.2024.06.004