biostudies-literatureUnknown16(1)Zhou R<h4>Background</h4>Previous evidence suggests that methionine (Met) consumption can promote placental angiogenesis and improve fetal survival. To investigate the mechanisms by which increased levels of Met as hydroxyl-Met (OHMet) improve placental function, forty sows were divided into four groups and fed either a control diet, or diets supplemented with 0.15% OHMet, 0.3% OHMet or 0.3% Met (n = 10). Placentas were collected immediately after expulsion, and extracted proteins were analyzed by tandem mass tag based quantitative proteomic analysis.<h4>Results</h4>We found that 0.15% OHMet consumption significantly increased placental vascular density compared with the control. Proteomic analysis identified 5,136 proteins, 87 of these were differentially expressed (P < 0.05, |fold change| > 1.Journal of animal science and biotechnology39https://www.ebi.ac.uk/biostudies/studies/S-EPMC11890719biostudies-literatureIncreased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows.PMC11890719Yuan PYang LZhuo YZhe LZhou RLin YFeng BChe LHua LFang ZZhang XXu SLai SHu LWuMercier YfalseIncreased maternal consumption of methionine as its hydroxyl analog improves placental angiogenesis and antioxidative capacity in sows.<h4>Background</h4>Previous evidence suggests that methionine (Met) consumption can promote placental angiogenesis and improve fetal survival. To investigate the mechanisms by which increased levels of Met as hydroxyl-Met (OHMet) improve placental function, forty sows were divided into four groups and fed either a control diet, or diets supplemented with 0.15% OHMet, 0.3% OHMet or 0.3% Met (n = 10). Placentas were collected immediately after expulsion, and extracted proteins were analyzed by tandem mass tag based quantitative proteomic analysis.<h4>Results</h4>We found that 0.15% OHMet consumption significantly increased placental vascular density compared with the control. Proteomic analysis identified 5,136 proteins, 87 of these were differentially expressed (P < 0.05, |fold change| > 1.2025-01-01T00:00:00Z2025 Mar2026-06-01T20:12:01.325Z2025-04-07T07:53:52.203ZS-EPMC118907194005778210.1186/s40104-025-01159-z