biostudies-literatureRajagopal VDeutsche ForschungsgemeinschaftDeutsche Forschungsgemeinschaft (German Research Foundation)Deutsche KrebshilfeBaden-Württemberg Stiftung (Baden-Württemberg Foundation)Center for Strategic Scientific Initiatives, National Cancer Institute (NCI Center for Strategic Scientific Initiatives)NCI NIH HHSBaden-Württemberg StiftungU.S. Department of Health & Human Services | National Institutes of Health (NIH)Center for Strategic Scientific Initiatives, National Cancer InstituteNIGMS NIH HHSU.S. Department of Health & Human Services | National Institutes of HealthDeutsche Krebshilfe (German Cancer Aid)2325https://www.ebi.ac.uk/biostudies/studies/S-EPMC11890761biostudies-literatureUnknown16(1)Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate of RNA. Inversely, RNA riboregulates the interactions and functions of the associated proteins. Dysregulation of ribonucleoprotein functions is linked to diseases such as cancer and neurological disorders. In dividing cells, RNA and RNA-binding proteins are present in mitotic structures, but their impact on cell division remains unclear. By applying the proteome-wide R-DeeP strategy to cells synchronized in mitosis versus interphase integrated with the RBP2GO knowledge, we provided an atlas of RNA-dependent proteins in cell division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 and TPX2 as unconventional RNA-binding proteins. KIFC1 was identified as a new substrate of AUNature communicationsAn atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions.PMC11890761P30 CA02310870113919BWST-ISF2019-027P30CA023108 DCC Core grantR35 GM119455DFG ZA 881/6-1 (project 541627224)R35GM119455Rajagopal VTheiss JKettenbach ANSeiler JCantarella SDiederichs SHerget FSchneider MWill RNasa IKaifer BKonig JZarnack KCaudron-Herger MKlostermann MHelm DfalseAn atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions.Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate of RNA. Inversely, RNA riboregulates the interactions and functions of the associated proteins. Dysregulation of ribonucleoprotein functions is linked to diseases such as cancer and neurological disorders. In dividing cells, RNA and RNA-binding proteins are present in mitotic structures, but their impact on cell division remains unclear. By applying the proteome-wide R-DeeP strategy to cells synchronized in mitosis versus interphase integrated with the RBP2GO knowledge, we provided an atlas of RNA-dependent proteins in cell division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 and TPX2 as unconventional RNA-binding proteins. KIFC1 was identified as a new substrate of AU2025-01-01T00:00:00Z2025 Mar2026-06-01T20:12:16.511Z2025-04-07T07:48:55.187ZS-EPMC118907614005747010.1038/s41467-025-57671-3