biostudies-literatureUnknown15Hankoua BBCassava is a crucial source of daily calorie intake for millions of people in sub-Saharan Africa (SSA) but has an inferior protein content. Despite numerous attempts utilizing both traditional and biotechnological methods, efforts to address protein deficiency in cassava have yet to meet with much success. We aim to leverage modern biotechnologies to enhance cassava's nutritional value by creating bioengineered cassava cultivars with increased protein and starch content. In this study, we utilized <i>Qui-Quine Starch</i> (<i>QQS</i>), a novel orphan gene unique to <i>Arabidopsis thaliana</i>, to develop transgenic cassava plants with increased protein and starch accumulation in their tissues. A total of 10 independent transgenic cassava lines expressing <i>QQS</i> were successfully regenerFrontiers in plant science1442324https://www.ebi.ac.uk/biostudies/studies/S-EPMC11891011biostudies-literatureConstitutive overexpression of &lt;i&gt;Qui-Quine Starch&lt;/i&gt; gene simultaneously improves starch and protein content in bioengineered cassava (&lt;i&gt;Manihot esculenta&lt;/i&gt; Crantz).PMC11891011Harun SHankoua BBGarcia RALigaba-Osena ADiao MAhlawat YKfalseConstitutive overexpression of &lt;i&gt;Qui-Quine Starch&lt;/i&gt; gene simultaneously improves starch and protein content in bioengineered cassava (&lt;i&gt;Manihot esculenta&lt;/i&gt; Crantz).Cassava is a crucial source of daily calorie intake for millions of people in sub-Saharan Africa (SSA) but has an inferior protein content. Despite numerous attempts utilizing both traditional and biotechnological methods, efforts to address protein deficiency in cassava have yet to meet with much success. We aim to leverage modern biotechnologies to enhance cassava's nutritional value by creating bioengineered cassava cultivars with increased protein and starch content. In this study, we utilized <i>Qui-Quine Starch</i> (<i>QQS</i>), a novel orphan gene unique to <i>Arabidopsis thaliana</i>, to develop transgenic cassava plants with increased protein and starch accumulation in their tissues. A total of 10 independent transgenic cassava lines expressing <i>QQS</i> were successfully regener2024-01-01T00:00:00Z20242026-06-01T10:32:31.523Z2025-04-05T11:17:52.289ZS-EPMC118910114006634710.3389/fpls.2024.1442324