biostudies-literatureUnknown11(4)de Annunzio SRThe growing global threat posed by microorganisms resistant to conventional antimicrobials underscores the urgent need for novel agents to control infections. The aim of this study was to evaluate the antimicrobial activity and biocompatibility of alpha-silver tungstate (α-Ag₂WO₄) nanoparticles (NPs) synthesized by the ultrasonic method. The NPs were characterized, and their antimicrobial activity was assessed against <i>Candida albicans</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i> using the broth microdilution method, determining the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Intracellular reactive oxygen species (ROS) production was detected by fluorescence using the CM-H₂DCFDA probe. CytotoxHeliyone42648https://www.ebi.ac.uk/biostudies/studies/S-EPMC11891672biostudies-literatureAntimicrobial activity and biocompatibility of alpha-silver tungstate nanoparticles.PMC11891672Assis MHenrique Ferreira Pereira de Oliveira VLima Moraes BVergani CEBarbugli PALongo Ede Annunzio SRfalseAntimicrobial activity and biocompatibility of alpha-silver tungstate nanoparticles.The growing global threat posed by microorganisms resistant to conventional antimicrobials underscores the urgent need for novel agents to control infections. The aim of this study was to evaluate the antimicrobial activity and biocompatibility of alpha-silver tungstate (α-Ag₂WO₄) nanoparticles (NPs) synthesized by the ultrasonic method. The NPs were characterized, and their antimicrobial activity was assessed against <i>Candida albicans</i>, <i>Staphylococcus aureus</i>, and <i>Escherichia coli</i> using the broth microdilution method, determining the minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). Intracellular reactive oxygen species (ROS) production was detected by fluorescence using the CM-H₂DCFDA probe. Cytotox2025-01-01T00:00:00Z2025 Feb2025-04-04T08:22:46.349Z2025-04-04T08:22:46.349ZS-EPMC118916724006604610.1016/j.heliyon.2025.e42648