biostudies-literatureUnknown17(9)Hu QThe diagnosis of metastatic and recurrent occult thyroid cancer presents a significant challenge. This study introduces a DNA-Mn hydrogel (M-TDH) that specifically targets thyroglobulin (Tg). This nanogel is loaded with paramagnetic Mn²⁺ for facilitating magnetic resonance (MR) imaging. As a cofactor of DNA polymerase, Mn²⁺ promotes the extension of long-strand DNA and forms Mn₂PPi nuclei with PPi^4- in the system. The synthesis of M-TDH is achieved through Mn₂PPi nucleation and growth with long-strand DNA acting as the structural framework. The X-scaffold functions as a junction point, thereby enhancing structural stability. The Tg aptamer sequence is incorporated into M-TDH, ensuring specific targeting of thyroid cancer cells. FurtherACS applied materials & interfaces13403-13414https://www.ebi.ac.uk/biostudies/studies/S-EPMC11891823biostudies-literatureManganese-Loaded pH-Responsive DNA Hydrogels Enable Tg-Guided Thyroid Tumor Targeted Magnetic Resonance Imaging.PMC11891823Tang ZLiu JWang DYHuang TWang RZhang XHu QRen AMing JfalseManganese-Loaded pH-Responsive DNA Hydrogels Enable Tg-Guided Thyroid Tumor Targeted Magnetic Resonance Imaging.The diagnosis of metastatic and recurrent occult thyroid cancer presents a significant challenge. This study introduces a DNA-Mn hydrogel (M-TDH) that specifically targets thyroglobulin (Tg). This nanogel is loaded with paramagnetic Mn²⁺ for facilitating magnetic resonance (MR) imaging. As a cofactor of DNA polymerase, Mn²⁺ promotes the extension of long-strand DNA and forms Mn₂PPi nuclei with PPi^4- in the system. The synthesis of M-TDH is achieved through Mn₂PPi nucleation and growth with long-strand DNA acting as the structural framework. The X-scaffold functions as a junction point, thereby enhancing structural stability. The Tg aptamer sequence is incorporated into M-TDH, ensuring specific targeting of thyroid cancer cells. Further2025-01-01T00:00:00Z2025 Mar2025-04-04T08:22:34.654Z2025-04-04T08:22:34.654ZS-EPMC118918233999696610.1021/acsami.4c19676