{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cheng X"],"funding":["HHS | National Institutes of Health","Elisabeth K. Harris Foundation Trust","HHS | National Institutes of Health (NIH)","NIDDK NIH HHS","NIDA NIH HHS","HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","HHS | NIH | National Institute of Neurological Disorders and Stroke","HHS | NIH | National Institute on Drug Abuse (NIDA)","HHS | NIH | National Institute on Drug Abuse","NINDS NIH HHS","NIGMS NIH HHS"],"pagination":["e2425526122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11892595"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(9)"],"pubmed_abstract":["Mimicking metabolic pathways on electrodes enables in vivo metabolite monitoring for decoding metabolism. Conventional in vivo sensors cannot accommodate underlying complex reactions involving multiple enzymes and cofactors, addressing only a fraction of enzymatic reactions for few metabolites. We devised a single-wall-carbon-nanotube-electrode architecture supporting tandem metabolic pathway-like reactions linkable to oxidoreductase-based electrochemical analysis, making a vast majority of metabolites detectable in vivo. This architecture robustly integrates cofactors, self-mediates reactions at maximum enzyme capacity, and facilitates metabolite intermediation/detection and interference inactivation through multifunctional enzymatic use. Accordingly, we developed sensors targeting 12 met"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Tandem metabolic reaction-based sensors unlock in vivo metabolomics."],"pmcid":["PMC11892595"],"funding_grant_id":["N/A","R33 DK128711","R35GM138241","5R21DK128711-02","R01DA047637","R01NS115537","UH3 NS119772","R01 DA047637","R01 NS115537","R35 GM138241","UH3NS119772","R21 DK128711"],"pubmed_authors":["Bahramian M","Lei L","Reyes BL","Lin S","Davis RW","Hojaiji H","Jelinek D","Emaminejad S","Cheng X","Wang J","Huang R","Patel P","Gromilina E","Forman S","Milla C","Zhang A","Yu KB","Puri S","Tan J","Ho C","Lu DC","Li Z","Zhu J","Yu LW","Matsumoto JH","Coller HA","Liu C","Voisin L","Hsiao EY"],"additional_accession":[]},"is_claimable":false,"name":"Tandem metabolic reaction-based sensors unlock in vivo metabolomics.","description":"Mimicking metabolic pathways on electrodes enables in vivo metabolite monitoring for decoding metabolism. Conventional in vivo sensors cannot accommodate underlying complex reactions involving multiple enzymes and cofactors, addressing only a fraction of enzymatic reactions for few metabolites. We devised a single-wall-carbon-nanotube-electrode architecture supporting tandem metabolic pathway-like reactions linkable to oxidoreductase-based electrochemical analysis, making a vast majority of metabolites detectable in vivo. This architecture robustly integrates cofactors, self-mediates reactions at maximum enzyme capacity, and facilitates metabolite intermediation/detection and interference inactivation through multifunctional enzymatic use. Accordingly, we developed sensors targeting 12 met","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Mar","modification":"2026-05-29T16:49:24.279Z","creation":"2025-04-04T02:21:55.809Z"},"accession":"S-EPMC11892595","cross_references":{"pubmed":["40014569"],"doi":["10.1073/pnas.2425526122"]}}