{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muneshige K"],"funding":["University of Aberdeen","Biotechnology and Biological Sciences Research Council","Japan Student Services Organization"],"pagination":["e202407021"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11893502"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["224(5)"],"pubmed_abstract":["Organelle biogenesis is fundamental to eukaryotic cell biology. Yeast signaling endosomes were recently identified as a signaling platform for the evolutionarily conserved Target of Rapamycin Complex 1 (TORC1) kinase complex. Despite the importance of signaling endosomes for TORC1-mediated control of cellular metabolism, how this organelle is generated has been a mystery. Here, we developed a system to induce synchronized de novo formation of signaling endosomes, enabling real-time monitoring of their biogenesis. Using this system, we identify vacuoles as a membrane source for newly formed signaling endosomes. Membrane supply from vacuoles is mediated by the CROP membrane-cutting complex, consisting of Atg18 PROPPIN and retromer subunits. The formation of signaling endosomes requires TORC1 activity, suggestive of a tightly regulated process. This study unveiled the first mechanistic principles and molecular participants of signaling endosome biogenesis."],"journal":["The Journal of cell biology"],"pubmed_title":["Vacuoles provide the source membrane for TORC1-containing signaling endosomes."],"pmcid":["PMC11893502"],"funding_grant_id":["BB/X018229/1","BB/V016334/1"],"pubmed_authors":["Hatakeyama R","Muneshige K"],"additional_accession":[]},"is_claimable":false,"name":"Vacuoles provide the source membrane for TORC1-containing signaling endosomes.","description":"Organelle biogenesis is fundamental to eukaryotic cell biology. Yeast signaling endosomes were recently identified as a signaling platform for the evolutionarily conserved Target of Rapamycin Complex 1 (TORC1) kinase complex. Despite the importance of signaling endosomes for TORC1-mediated control of cellular metabolism, how this organelle is generated has been a mystery. Here, we developed a system to induce synchronized de novo formation of signaling endosomes, enabling real-time monitoring of their biogenesis. Using this system, we identify vacuoles as a membrane source for newly formed signaling endosomes. Membrane supply from vacuoles is mediated by the CROP membrane-cutting complex, consisting of Atg18 PROPPIN and retromer subunits. The formation of signaling endosomes requires TORC1 activity, suggestive of a tightly regulated process. This study unveiled the first mechanistic principles and molecular participants of signaling endosome biogenesis.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 May","modification":"2026-05-29T14:33:47.436Z","creation":"2025-04-05T23:53:10.921Z"},"accession":"S-EPMC11893502","cross_references":{"pubmed":["40052923"],"doi":["10.1083/jcb.202407021"]}}