biostudies-literatureLi SPrimary Research & Development Plan of Shandong Province, ChinaYoung Talent of Lifting engineering for Science and Technology in Shandong，ChinaNatural Science Foundation of Shandong ProvinceNational Natural Science Foundation of China8182https://www.ebi.ac.uk/biostudies/studies/S-EPMC11894197biostudies-literatureUnknown15(1)Rose (Rosa hybrida) is one of the most important ornamental and perfume industry crops worldwide, both economically and culturally. Abiotic stresses, such as high temperature and salt are crucial factors influencing the quality of roses. In this study, RhHsf24 was isolated from rose (R. hybrida 'Samantha'), which encodes 295 amino acids (aa). Sequence comparison with members of Arabidopsis Hsfs family revealed that this gene is most closely related to AtHsfB1; phylogenetic tree analysis with proteins from other species showed that it clusters with R. rugosa (RrHSF24), Fragaria vesca (FvHSFB1a) and Argentina anserina (AaHSF24), which are the closest relatives and belong to the class B heat shock transcription factors. RhHsf24 was localized in the nucleus. The qRT-PCR results indicated that Scientific reportsCloning and expression analysis of RhHsf24 gene in Rose (Rosa hybrida).PMC11894197ZR2024MC176SDAST2024QTA0012022LZGCQY00932102427ZR2021QC1302024LZGC027Dong FCao MLi SZhu JHu ZWang CSun YfalseCloning and expression analysis of RhHsf24 gene in Rose (Rosa hybrida).Rose (Rosa hybrida) is one of the most important ornamental and perfume industry crops worldwide, both economically and culturally. Abiotic stresses, such as high temperature and salt are crucial factors influencing the quality of roses. In this study, RhHsf24 was isolated from rose (R. hybrida 'Samantha'), which encodes 295 amino acids (aa). Sequence comparison with members of Arabidopsis Hsfs family revealed that this gene is most closely related to AtHsfB1; phylogenetic tree analysis with proteins from other species showed that it clusters with R. rugosa (RrHSF24), Fragaria vesca (FvHSFB1a) and Argentina anserina (AaHSF24), which are the closest relatives and belong to the class B heat shock transcription factors. RhHsf24 was localized in the nucleus. The qRT-PCR results indicated that 2025-01-01T00:00:00Z2025 Mar2025-04-26T02:50:38.17Z2025-04-06T10:35:32.868ZS-EPMC118941974006504010.1038/s41598-025-93421-7