biostudies-literatureUnknown31(5)Zhang HLncPrep + 96kb is a long non‑coding RNA expressed in murine granulosa cells. The 2.2-kb fragment of lncPrep + 96kb inhibits aromatase expression and estrogen secretion in ovarian granulosa cells. In the present study, lncPrep + 96kb‑knockout (KO) mice were generated, and significant ovarian fibrosis and reduced female fertility through fertility monitoring and superovulation. The augmentation of ovarian fibrosis was observed by Sirius red staining and western blot and <i>RT‑qPCR</i>. Notably, lncPrep + 96kb was identified in conserved non‑coding sequences adjacent to the prolyl oligopeptidase (POP) gene. Furthermore, POP expression was shown to be reduced in lncPrep + 96kb‑KO mice, whereas overexpression of lncPrep + 96kb increased POP expression. Further studies revealed that POP regulateMolecular medicine reports113https://www.ebi.ac.uk/biostudies/studies/S-EPMC11894593biostudies-literatureLncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression.PMC11894593Li XLiu JHan QFan XHuang YXiao LWang SZhang HJia YZhang CWang JfalseLncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression.LncPrep + 96kb is a long non‑coding RNA expressed in murine granulosa cells. The 2.2-kb fragment of lncPrep + 96kb inhibits aromatase expression and estrogen secretion in ovarian granulosa cells. In the present study, lncPrep + 96kb‑knockout (KO) mice were generated, and significant ovarian fibrosis and reduced female fertility through fertility monitoring and superovulation. The augmentation of ovarian fibrosis was observed by Sirius red staining and western blot and <i>RT‑qPCR</i>. Notably, lncPrep + 96kb was identified in conserved non‑coding sequences adjacent to the prolyl oligopeptidase (POP) gene. Furthermore, POP expression was shown to be reduced in lncPrep + 96kb‑KO mice, whereas overexpression of lncPrep + 96kb increased POP expression. Further studies revealed that POP regulate2025-01-01T00:00:00Z2025 May2026-05-29T14:33:06.092Z2025-04-06T03:20:13.713ZS-EPMC118945934001714810.3892/mmr.2025.13478