biostudies-literatureUnknown6(6)Hu TQ fever is a zoonotic disease caused by infection with <i>Coxiella burnetii</i> (<i>C. burnetii</i>). Due to its atypical symptoms and the absence of specific detection methods, Q fever is underdiagnosed commonly. Herein, we report a case of Q fever confirmed by metagenomic next-generation sequencing (mNGS) in March 2024 in Guangdong Province, China. The patient initially experienced fever and was admitted to hospital six days later. Despite a series of laboratory tests conducted at the hospital, the pathogen remained undetermined. Ten days after admission, mNGS revealed that the patient was infected with <i>C. burnetii</i>. The patient subsequently underwent treatment with doxycycline and recovered well. Epidemiological investigation revealed that the patient had been exposed to sheep infBiosafety and health337-340https://www.ebi.ac.uk/biostudies/studies/S-EPMC11895022biostudies-literatureQ fever diagnosed using metagenomic next-generation sequencing in Guangdong Province, China.PMC11895022Zhou MHu TZhang MCheng YZhuang YLiu YLi XWang PTan XZhang XDeng AWan JCheng LKang MZong JfalseQ fever diagnosed using metagenomic next-generation sequencing in Guangdong Province, China.Q fever is a zoonotic disease caused by infection with <i>Coxiella burnetii</i> (<i>C. burnetii</i>). Due to its atypical symptoms and the absence of specific detection methods, Q fever is underdiagnosed commonly. Herein, we report a case of Q fever confirmed by metagenomic next-generation sequencing (mNGS) in March 2024 in Guangdong Province, China. The patient initially experienced fever and was admitted to hospital six days later. Despite a series of laboratory tests conducted at the hospital, the pathogen remained undetermined. Ten days after admission, mNGS revealed that the patient was infected with <i>C. burnetii</i>. The patient subsequently underwent treatment with doxycycline and recovered well. Epidemiological investigation revealed that the patient had been exposed to sheep inf2024-01-01T00:00:00Z2024 Dec2025-04-04T02:54:20.478Z2025-04-04T02:54:20.478ZS-EPMC118950224007898110.1016/j.bsheal.2024.11.003