{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yeo HR"],"funding":["Ministry of Science and ICT","Yonsei University College of Medicine","National Research Foundation of Korea"],"pagination":["12-24"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11895090"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["63(1)"],"pubmed_abstract":["Intraflagellar transport (IFT) particles, a multi-protein apparatus composed of complex A and B, are known to be involved in homeostasis of flagella formation. IFT particles have recently become an interesting topic in Giardia lamblia, which has 4 pairs of flagella. In this experiment, we examined the function of giardial IFT components. When 7 components (IFT121, 140, 20, 46, 52, 81, and 88) of IFT were expressed in Giardia trophozoites as a tagged form with mNeonGreen, all of them were found in both flagella pores and cytoplasmic axonemes. In addition, motor proteins for IFT particles (kinesin-13 and kinesin-2b), were localized to a median body and cytoplasmic flagella, respectively. The CRISPRi-mediated knockdown of IFT88 significantly affected the lengths of all 4 flagella compared to "],"journal":["Parasites, hosts and diseases"],"pubmed_title":["Giardia intraflagellar transport protein 88 is involved in flagella formation."],"pmcid":["PMC11895090"],"funding_grant_id":["6-2022-0043","2023R1A2C1002475"],"pubmed_authors":["Shin MY","Yeo HR","Kim J","Park SJ"],"additional_accession":[]},"is_claimable":false,"name":"Giardia intraflagellar transport protein 88 is involved in flagella formation.","description":"Intraflagellar transport (IFT) particles, a multi-protein apparatus composed of complex A and B, are known to be involved in homeostasis of flagella formation. IFT particles have recently become an interesting topic in Giardia lamblia, which has 4 pairs of flagella. In this experiment, we examined the function of giardial IFT components. When 7 components (IFT121, 140, 20, 46, 52, 81, and 88) of IFT were expressed in Giardia trophozoites as a tagged form with mNeonGreen, all of them were found in both flagella pores and cytoplasmic axonemes. In addition, motor proteins for IFT particles (kinesin-13 and kinesin-2b), were localized to a median body and cytoplasmic flagella, respectively. The CRISPRi-mediated knockdown of IFT88 significantly affected the lengths of all 4 flagella compared to ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Feb","modification":"2026-06-01T12:52:18.352Z","creation":"2025-04-04T13:11:19.638Z"},"accession":"S-EPMC11895090","cross_references":{"pubmed":["40045677"],"doi":["10.3347/PHD.24064"]}}