biostudies-literatureYeo HRMinistry of Science and ICTYonsei University College of MedicineNational Research Foundation of Korea12-24https://www.ebi.ac.uk/biostudies/studies/S-EPMC11895090biostudies-literatureUnknown63(1)Intraflagellar transport (IFT) particles, a multi-protein apparatus composed of complex A and B, are known to be involved in homeostasis of flagella formation. IFT particles have recently become an interesting topic in Giardia lamblia, which has 4 pairs of flagella. In this experiment, we examined the function of giardial IFT components. When 7 components (IFT121, 140, 20, 46, 52, 81, and 88) of IFT were expressed in Giardia trophozoites as a tagged form with mNeonGreen, all of them were found in both flagella pores and cytoplasmic axonemes. In addition, motor proteins for IFT particles (kinesin-13 and kinesin-2b), were localized to a median body and cytoplasmic flagella, respectively. The CRISPRi-mediated knockdown of IFT88 significantly affected the lengths of all 4 flagella compared to Parasites, hosts and diseasesGiardia intraflagellar transport protein 88 is involved in flagella formation.PMC118950906-2022-00432023R1A2C1002475Shin MYYeo HRKim JPark SJfalseGiardia intraflagellar transport protein 88 is involved in flagella formation.Intraflagellar transport (IFT) particles, a multi-protein apparatus composed of complex A and B, are known to be involved in homeostasis of flagella formation. IFT particles have recently become an interesting topic in Giardia lamblia, which has 4 pairs of flagella. In this experiment, we examined the function of giardial IFT components. When 7 components (IFT121, 140, 20, 46, 52, 81, and 88) of IFT were expressed in Giardia trophozoites as a tagged form with mNeonGreen, all of them were found in both flagella pores and cytoplasmic axonemes. In addition, motor proteins for IFT particles (kinesin-13 and kinesin-2b), were localized to a median body and cytoplasmic flagella, respectively. The CRISPRi-mediated knockdown of IFT88 significantly affected the lengths of all 4 flagella compared to 2025-01-01T00:00:00Z2025 Feb2026-06-01T12:52:18.352Z2025-04-04T13:11:19.638ZS-EPMC118950904004567710.3347/PHD.24064