{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Burton NR"],"funding":["Tobacco-Related Disease Research Program","Defense Advanced Research Projects Agency","National Cancer Institute","NCI NIH HHS","NIGMS NIH HHS","Arnold and Mabel Beckman Foundation"],"pagination":["21303-21318"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11895830"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["145(39)"],"pubmed_abstract":["Mass spectrometry-based chemoproteomics has emerged as an enabling technology for functional biology and drug discovery. To address limitations of established chemoproteomics workflows, including cumbersome reagent synthesis and low throughput sample preparation, here, we established the silane-based cleavable isotopically labeled proteomics (sCIP) method. The sCIP method is enabled by a high yielding and scalable route to dialkoxydiphenylsilane fluorenylmethyloxycarbonyl (DADPS-Fmoc)-protected amino acid building blocks, which enable the facile synthesis of customizable, isotopically labeled, and chemically cleavable biotin capture reagents. sCIP is compatible with both MS1- and MS2-based quantitation, and the sCIP-MS2 method is distinguished by its click-assembled isobaric tags in which "],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Solid-Phase Compatible Silane-Based Cleavable Linker Enables Custom Isobaric Quantitative Chemoproteomics."],"pmcid":["PMC11895830"],"funding_grant_id":["D19AP00041","R01 GM094231","DP2 GM146246","T32 GM067555","T32 CA140044","T31DT1800","U24 CA271037","U24CA271037"],"pubmed_authors":["Polasky DA","Burton NR","Backus KM","Shikwana F","Geiszler DJ","Yan T","Nesvizhskii AI","Ofori S","Veiga Leprevost FD"],"additional_accession":[]},"is_claimable":false,"name":"Solid-Phase Compatible Silane-Based Cleavable Linker Enables Custom Isobaric Quantitative Chemoproteomics.","description":"Mass spectrometry-based chemoproteomics has emerged as an enabling technology for functional biology and drug discovery. To address limitations of established chemoproteomics workflows, including cumbersome reagent synthesis and low throughput sample preparation, here, we established the silane-based cleavable isotopically labeled proteomics (sCIP) method. The sCIP method is enabled by a high yielding and scalable route to dialkoxydiphenylsilane fluorenylmethyloxycarbonyl (DADPS-Fmoc)-protected amino acid building blocks, which enable the facile synthesis of customizable, isotopically labeled, and chemically cleavable biotin capture reagents. sCIP is compatible with both MS1- and MS2-based quantitation, and the sCIP-MS2 method is distinguished by its click-assembled isobaric tags in which ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Oct","modification":"2026-06-02T19:16:48.358Z","creation":"2025-04-04T02:50:38.204Z"},"accession":"S-EPMC11895830","cross_references":{"pubmed":["37738129"],"doi":["10.1021/jacs.3c05797"]}}