{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["38(4)"],"submitter":["Peng LY"],"pubmed_abstract":["Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa. M2 macrophage polarization can reduce inflammation and repair tissue injury during AR development. Studies have substantiated the involvement of miRNAs in AR pathogenesis. Herein, the molecular mechanism of miR-214-3p in AR development was explored. To mimic the AR environment, ovalbumin (OVA) was used to treat macrophages. MiR-214-3p and glycogen synthase kinase 3 beta (GSK3B) expression in nasal mucus tissues and macrophages was assessed by RT-qPCR. The M2 phenotypic signature of CD206 in macrophages was assessed by flow cytometry. The protein expression of GSK3B and M2 macrophage markers (ARG-1 and IL-10) was evaluated by western blotting. The correlation between miR-214-3p and GSK3B was validated by a lucifer"],"journal":["The Kaohsiung journal of medical sciences"],"pagination":["347-356"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11896522"],"repository":["biostudies-literature"],"pubmed_title":["MicroRNA-214-3p facilitates M2 macrophage polarization by targeting GSK3B."],"pmcid":["PMC11896522"],"pubmed_authors":["Deng KB","Peng LY","Li BB","Wang WG"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-214-3p facilitates M2 macrophage polarization by targeting GSK3B.","description":"Allergic rhinitis (AR) is a chronic inflammatory disease of the nasal mucosa. M2 macrophage polarization can reduce inflammation and repair tissue injury during AR development. Studies have substantiated the involvement of miRNAs in AR pathogenesis. Herein, the molecular mechanism of miR-214-3p in AR development was explored. To mimic the AR environment, ovalbumin (OVA) was used to treat macrophages. MiR-214-3p and glycogen synthase kinase 3 beta (GSK3B) expression in nasal mucus tissues and macrophages was assessed by RT-qPCR. The M2 phenotypic signature of CD206 in macrophages was assessed by flow cytometry. The protein expression of GSK3B and M2 macrophage markers (ARG-1 and IL-10) was evaluated by western blotting. The correlation between miR-214-3p and GSK3B was validated by a lucifer","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-07-26T03:06:58.847Z","creation":"2025-07-26T03:06:58.847Z"},"accession":"S-EPMC11896522","cross_references":{"pubmed":["35005835"],"doi":["10.1002/kjm2.12487"]}}