biostudies-literatureXu KNIDA NIH HHSe70267https://www.ebi.ac.uk/biostudies/studies/S-EPMC11896887biostudies-literatureUnknown15(3)<h4>Background</h4>The HIV-1 reservoir in CD4+ T cells (HR_CD4) pose a major challenge to curing HIV, with many of its mechanisms still unclear. HIV-1 DNA integration and immune responses may alter the host's epigenetic landscape, potentially silencing HIV-1 replication.<h4>Methods</h4>This study used bisulphite capture DNA methylation sequencing in CD4+ T cells from the blood of 427 virally suppressed women with HIV to identify differentially methylated sites and regions associated with HR_CD4.<h4>Results</h4>The average total HR_CD4 size was 1409 copies per million cells, with most proviruses defective and only a small proportion intact. The study identified 245 differentially methylated CpG sites and 85 regions linked to HR_CD4 size, with 52% of sClinical and translational medicineAberrant DNA methylation of genes regulating CD4+ T cell HIV-1 reservoir in women with HIV.PMC11896887R01DA047820R01DA052846R01DA047063R01DA061926Xu KAsam KKuniholm MHArchin NMPage GPValizadeh ATopper EFCohen MHAouizerat BEDeHovitz JTien PCMarconi VCZhang XJohnson EOLahiri CDMartinez CHancock DBKonkle-Parker DQuach BCKassaye SGfalseAberrant DNA methylation of genes regulating CD4+ T cell HIV-1 reservoir in women with HIV.<h4>Background</h4>The HIV-1 reservoir in CD4+ T cells (HR_CD4) pose a major challenge to curing HIV, with many of its mechanisms still unclear. HIV-1 DNA integration and immune responses may alter the host's epigenetic landscape, potentially silencing HIV-1 replication.<h4>Methods</h4>This study used bisulphite capture DNA methylation sequencing in CD4+ T cells from the blood of 427 virally suppressed women with HIV to identify differentially methylated sites and regions associated with HR_CD4.<h4>Results</h4>The average total HR_CD4 size was 1409 copies per million cells, with most proviruses defective and only a small proportion intact. The study identified 245 differentially methylated CpG sites and 85 regions linked to HR_CD4 size, with 52% of s2025-01-01T00:00:00Z2025 Mar2025-04-04T01:41:24.005Z2025-04-04T01:41:24.005ZS-EPMC118968874007000910.1002/ctm2.70267